According to the Enzymatic Assay Test Procedure, a solution should be prepared immediately before use, containing 500–1000 Kunitz units/ml in cold deionized water. More information can be found in the document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/769/284/d8764enz.pdf
D8764
Deoxyribonuclease II from bovine spleen
Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein
Sinonimo/i:
DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase
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30000 UNITS
CHF 141.00
150000 UNITS
CHF 496.00
300000 UNITS
CHF 897.00
CHF 141.00
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Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-801-8
MDL number:
Vai a
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Permettici di aiutartiNome del prodotto
Deoxyribonuclease II from bovine spleen, Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein
biological source
bovine spleen
type
Type V
form
essentially salt-free, lyophilized powder
specific activity
≥1,000 units/mg protein
mol wt
38 kDa
concentration
≥50% protein
technique(s)
DNA extraction: suitable
tissue culture: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
foreign activity
RNase ≤0.1%
storage temp.
−20°C
Quality Level
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Categorie correlate
1 of 4
Questo articolo | D4138 | D4513 | D2821 |
|---|---|---|---|
| technique(s) DNA extraction: suitable, tissue culture: suitable | technique(s) - | technique(s) DNA purification: suitable | technique(s) DNA extraction: suitable |
| specific activity ≥1,000 units/mg protein | specific activity 2,000-6,000 Kunitz units/mg protein (biuret) | specific activity ≥2,000 units/mg protein | specific activity ≥4,000 units/mg protein |
| biological source bovine spleen | biological source - | biological source bovine pancreas | biological source bovine |
| concentration ≥50% protein | concentration - | concentration - | concentration - |
| suitability suitable for molecular biology | suitability - | suitability suitable for molecular biology | suitability suitable for molecular biology |
| form essentially salt-free, lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
Application
Deoxyribonuclease II from bovine spleen has been used:
Biochem/physiol Actions
Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.[5]
Other Notes
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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K Nitta et al.
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 114(2), 119-128 (1994-02-01)
Deoxyribonucleases from eggs and the liver of Xenopus laevis were partially purified by DEAE-cellulose and heparin-Sepharose affinity column chromatographies. The fractions having egg and liver DNase activities were eluted on high performance liquid chromatography through TSK gel G3000SW at the
Dopamine D2 receptor stimulation differentially affects voltage-activated calcium channels in rat pituitary melanotropic cells.
Keja J A, et al.
The Journal of Physiology, 450(1), 409-435 (1992)
S Ingebrandt et al.
Biosensors & bioelectronics, 16(7-8), 565-570 (2001-09-07)
An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described
Therese Ostberg et al.
Arthritis and rheumatism, 62(10), 2963-2972 (2010-06-10)
High mobility group box chromosomal protein 1 (HMGB-1) is a DNA binding nuclear protein that can be released from dying cells and activated myeloid cells. Extracellularly, HMGB-1 promotes inflammation. Clinical and experimental studies demonstrate that HMGB-1 is a pathogenic factor
K Tempel
Arzneimittel-Forschung, 30(7), 1119-1123 (1980-01-01)
The influence of three mucopolysaccharide-polysulfuric acid-esters (MPS) of different molecular weight on DNAase II-activity as well as on nucleic acid and protein synthesis of lymphocytes of rat spleen has been investigated in vitro. The results are summarized as follows: 1.
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