E0885
Enterokinase from porcine intestine
lyophilized powder, ≥100 units/mg protein
Sinonimo/i:
Enteropeptidase
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
40 units | Per conoscere la disponibilità, visualizza il carrello | CHF 243.00 |
200 units | Per conoscere la disponibilità, visualizza il carrello | CHF 993.00 |
Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-761-1
NACRES:
NA.54
MDL number:
Numero CE:
Specific activity:
≥100 units/mg protein
Servizio Tecnico
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Permettici di aiutartiform
lyophilized powder
Quality Segment
specific activity
≥100 units/mg protein
mol wt
150 kDa
purified by
chromatography
composition
Protein, ≥20% Lowry
foreign activity
aminopeptidase ≤1.5%, trypsin ≤1%, free
storage temp.
−20°C
Application
The enzyme from Sigma has been used to activate zymogens in order to detect trypsin activity. The study to investigated the structural and evolutionary consequences of unpaired cysteines in trypsinogen. The product has been used to measure trypsin while studying the effect of pesticide induced alterations in gene expression in the lobster, Homarus americanus The enzyme from Sigma has been used to develop a novel assay for measuring levels of lipid-free apoA-I in the presence of lipid-bound apoA-I. Enteropeptidase can specifically cleave human lipid-free apoA-I but not its lipid-bound form resulting in an N-terminal fragment of 22 kDa. It has also been used in a study to examine the effect of calcium and phytic acid on the activation of trypsinogen and the stability of trypsin.
Enterokinase from porcine intestine has been used in a study to investigate complementary DNA cloning and sequencing of rat enteropeptidase. Enterokinase from porcine intestine has also been used to learn more about the insulinotropic region of the gastric inhibitory polypeptide.
Biochem/physiol Actions
Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.
Physical form
Lyophilized powder containing sodium phosphate buffer salts
Other Notes
One unit will produce 1.0 nanomole of trypsin from trypsinogen per min at pH 5.6 at 25 °C.
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Questo articolo | |||
|---|---|---|---|
| description lyophilized powder, ≥100 units/mg protein | description ≥0.5 units/mg solid | description powder | description BRP, European Pharmacopoeia (EP) Reference Standard |
| specific activity ≥100 units/mg protein | specific activity ≥0.5 units/mg solid | specific activity - | specific activity - |
| form lyophilized powder | form salt-free, lyophilized powder | form powder | form - |
| mol wt 150 kDa | mol wt 150 kDa | mol wt 42.3 kDa (+/- 6.4 kDa) | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. - |
| foreign activity trypsin ≤1%, free, aminopeptidase ≤1.5% | foreign activity - | foreign activity - | foreign activity - |
| purified by chromatography | purified by - | purified by - | purified by - |
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Classe di stoccaggio
11 - Combustible Solids
Che succede?
WGK 3
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
DPI (dispositivi di protezione individuale)
Eyeshields, Gloves, type N95 (US)
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