The Positive Control functions as a system control to verify the functionality of the kit components. The resulting OD will not be utilized in any calculations. Any signal within the standard curve range that demonstrates competition in the assay indicates that the assay is performing as anticipated.
RAB0010
Angiotensin II EIA Kit
for serum, plasma, culture supernatant and cell lysates
Sinonimo/i:
Angiotensinogen, Serpin A8
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1 kit | Per conoscere la disponibilità, visualizza il carrello | CHF 426.00 |
Informazioni su questo articolo
NACRES:
NA.32
UNSPSC Code:
12352204
eCl@ss:
32160701
CHF 426.00
Per conoscere la disponibilità, visualizza il carrello
Servizio Tecnico
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Permettici di aiutartispecies reactivity
mouse, human, rat
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, enzyme immunoassay: suitable
input
sample type cell lysate
sample type culture supernatant(s)
sample type serum
sample type plasma
assay range
inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 2.62 pg/mL
standard curve range: 0.1-1000 ng/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... AGT(183)
General description
The Angiotensin II Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Angiotensin II peptide based on the principle of Competitive Enzyme Immunoassay. EIA-ANGII-1 can theoretically detect all active angiotensins, including ANGI, ANGII, ANGIII and ANGIV. However, it does not detect inactive angiotensinogen.
Immunogen
Angiotensin II, synthetic peptide
Application
For research use only. Not for use in diagnostic procedures.
Angiotensin II EIA Kit has been used for analyzing circulating and serum levels of angiotensin II (Ang II) in mice and rats respectively.
Angiotensin II EIA Kit has been used for analyzing circulating and serum levels of angiotensin II (Ang II) in mice and rats respectively.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
Study on in vitro model shows that mechanical stretch induced Ang II release plays a vital role in mediating stretch-induced hypertrophic response. Ang II is implicated in maintaining blood pressure and has an important role in hypertension and in the pathophysiology of cardiovascular disorders. Ang II also has an essential role in growth induction, cell migration and mitosis of vascular smooth muscle cells. It enhances the production of collagen type I and III in fibroblasts, that results in thickening of the vascular wall and myocardium, and fibrosis. Variation in Ang II gene increases the risk of myocardial infarction, hypertension and left ventricular hypertrophy. Ang II expression is associated with heart failure, cardiac remodeling, diabetes, and the proliferative and inflammatory responses to arterial injury. The pharmacological agents such as angiotensin II-converting enzyme inhibitors or angiotensin II receptor antagonists can be used for treating Ang II-induced hypertension and congestive heart failure.
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Questo articolo | |||
|---|---|---|---|
| description for serum, plasma, culture supernatant and cell lysates | description for cell culture supernatants, plasma, and serum samples | description Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution | description Native angiotensinogen from human plasma that is cleaved by renin to generate angiotensin I. It is a part of the renin-angiotensin system that regulates blood pressure and fluid balance. |
| species reactivity mouse, human, rat | species reactivity human | species reactivity human | species reactivity - |
| detection method colorimetric | detection method colorimetric | detection method - | detection method - |
| Gene Information human ... AGT(183) | Gene Information human ... AGT(183) | Gene Information human ... AGT(183) | Gene Information human ... AGT(183) |
| technique(s) ELISA: suitable, enzyme immunoassay: suitable | technique(s) ELISA: suitable | technique(s) immunohistochemistry: 1:20-1:50, immunoblotting: 0.04-0.4 μg/mL | technique(s) cell based assay: suitable |
| assay range inter-assay cv: <15% | assay range inter-assay cv: <12% | assay range - | assay range - |
| input sample type cell lysate | input sample type serum sample(s) | input - | input - |
Parola di avvertenza
Danger
Codici di pericolo
Avvertenze di sicurezza
Hazard Classifications
Resp. Sens. 1
Classe di stoccaggio
10 - Combustible liquids
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What is the expected response for the positive control in the Human Angiotensin II EIA (RAB0010)?
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