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S8409

Superoxide Dismutase from bovine liver

Name: Superoxide Dismutase from bovine liver
Brand: SIGMA
Price: 48.2 CHF

ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)

Sinonimo/i:

SOD, Superoxide: superoxide oxidoreductase

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Voi/SKU	Disponibilità	Prezzo
3000 units	Per conoscere la disponibilità, visualizza il carrello	CHF 48.20
15000 units	Per conoscere la disponibilità, visualizza il carrello	CHF 131.00
30000 units	Per conoscere la disponibilità, visualizza il carrello	CHF 219.00

Informazioni su questo articolo

Numero CAS:

9054-89-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-943-0

MDL number:

MFCD00132404

Numero CE:

1.15.1.1 (BRENDA, IUBMB)

Specific activity:

2,000-6,000 units/mg protein (biuret)

CHF 48.20

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Proprietà

form

ammonium sulfate suspension

Quality Segment

200

specific activity

2,000-6,000 units/mg protein (biuret)

mol wt

32.5 kDa

UniProt accession no.

P00442

storage temp.

2-8°C

Gene Information

cow ... SOD1(281495)

Descrizione

General description

Research area: Cell Signaling

Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals, and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.

Application

Superoxide Dismutase from bovine liver has been used:

for measuring mitochondrial hydrogen peroxide production in skeletal muscle
as a positive control in Cu(II) binding stability assay
as a positive control in superoxide dismutase assay
in a study to assess the inactivation of endothelial-derived relaxing factor by oxidized lipoproteins.
in a study to investigate the role of hydrogen peroxide in the cytotoxicity of the xanthine/xanthine oxidase system.

Biochem/physiol Actions

Superoxide Dismutase catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It plays a critical role in the defense of cells against the toxic effects of oxygen radicals. It competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Physical form

Suspension in 3.8 M (NH₄)₂SO₄, pH 7.0

Analysis Note

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Other Notes

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀of 0.025 ± 0.005 per min.

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Questo articolo	S8160	S5395	S9697
Sigma-Aldrich S8409 Superoxide Dismutase from bovine liver	Sigma-Aldrich S8160 Superoxide Dismutase from bovine liver	Sigma-Aldrich S5395 Superoxide Dismutase from bovine erythrocytes	Sigma-Aldrich S9697 Superoxide Dismutase bovine
description ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)	description lyophilized powder, ≥1500 units/mg protein	description ≥3,000 units/mg protein, BioReagent, lyophilized powder, suitable for cell culture	description recombinant, expressed in E. coli, lyophilized powder, ≥2500 units/mg protein, ≥90% (SDS-PAGE)
Gene Information cow ... SOD1(281495)	Gene Information cow ... SOD1(281495)	Gene Information -	Gene Information -
specific activity 2,000-6,000 units/mg protein (biuret)	specific activity ≥1500 units/mg protein	specific activity ≥3,000 units/mg protein	specific activity ≥2500 units/mg protein
form ammonium sulfate suspension	form lyophilized powder	form lyophilized powder	form lyophilized powder
mol wt 32.5 kDa	mol wt 32.5 kDa	mol wt 32.5 kDa	mol wt -
UniProt accession no. P00442	UniProt accession no. P00442	UniProt accession no. -	UniProt accession no. P00442
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Pittogrammi

GHS08

Parola di avvertenza

Danger

Codici di pericolo

H334

Avvertenze di sicurezza

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Classe di stoccaggio

10 - Combustible liquids

Che succede?

WGK 1

Punto di infiammabilità (°F)

Not applicable

Punto di infiammabilità (°C)

Not applicable

DPI (dispositivi di protezione individuale)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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