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SEQXE

SeqPlex DNA Amplification Kit

For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

Sinonimo/i:

SeqPlex Enhanced DNA Amplification Kit, WGA kit, whole genome amplification kit

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Cambia visualizzazione
Taglio della confezioneSKUDisponibilitàPrezzo
10 reactions
Per conoscere la disponibilità, visualizza il carrello
CHF 349.00
50 reactions
Per conoscere la disponibilità, visualizza il carrello
CHF 1’480.00
500 reactions
Per conoscere la disponibilità, visualizza il carrello
CHF 10’330.00

Informazioni su questo articolo

NACRES:
NA.55
UNSPSC Code:
41121800

CHF 349.00


Per conoscere la disponibilità, visualizza il carrello

Servizio Tecnico
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Quality Level

technique(s)

whole genome amplification: suitable

dilution

(WGA)

input

purified DNA

shipped in

wet ice

storage temp.

−20°C

General description

SeqPlex Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina®, SOLiD, or 454 sequencing workflows.

Application

SeqPlex DNA Amplification Kit has been used for whole genome amplification.

Features and Benefits

  • Random priming technology amplifies fragmented DNA such as ChIP or FFPE
  • Facilitates sequencing from as little as 100 pg of ChIP DNA
  • Enhanced primers for complete genome coverage, minimal sequence bias, primer removal, and amplicon size ideal for next gen sequencing
  • Compatible with Illumina®, SOLiD, or 454 library prep for next generation sequencing

Other Notes

SEQXE-500RXN is manufactured on-demand. Contact technical services at [email protected] for more information.

Legal Information

Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC

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Questo articolo
SEQRWGA2WGA4
technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

dilution

(WGA)

dilution

(WTA)

dilution

(WGA)

dilution

(WGA)

input

purified DNA

input

purified RNA

input

purified DNA

input

purified DNA

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice


I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS & Determinazione del prezzo

  • Water, Nuclease-Free Water, for Molecular Biology
    SDS

  • Library Preparation Buffer
    SDS

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Classe di stoccaggio

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3



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Certificati d'analisi (COA)

Lot/Batch Number

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Protocolli

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

Contenuto correlato


Xiang Yu et al.
Genome research, 27(7), 1238-1249 (2017-04-08)
Type II topoisomerases orchestrate proper DNA topology, and they are the targets of anti-cancer drugs that cause treatment-related leukemias with balanced translocations. Here, we develop a high-throughput sequencing technology to define TOP2 cleavage sites at single-base precision, and use the
Syuzo Kaneko et al.
Cancers, 13(9) (2021-05-01)
Although chromatin immunoprecipitation and next-generation sequencing (ChIP-seq) using formalin-fixed paraffin-embedded tissue (FFPE) has been reported, it remained elusive whether they retained accurate transcription factor binding. Here, we developed a method to identify the binding sites of the insulator transcription factor
Isolation and sequencing of active origins of DNA replication by nascent strand capture and release (NSCR).
Kunnev D, et al.
Journal of biological methods, 2(4) (2015)



Numero articolo commerciale globale

SKUGTIN
SEQXE-10RXN04061837018138
SEQXE-50RXN04061837018176
SEQXE-500RXN04061837018145

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