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Merck

Scheda delle specifiche

Nome del prodotto
MystiCq® microRNA cDNA Synthesis Mix

Numero di prodotto
mirrt

Marchio del prodotto
sigma

Numero CAS

Peso molecolare

Test

Specifiche

qScript microRNA cDNA Synthesis KitMust have detection of microRNA from 100 ng to 10 pg.Correlation of determination (R2) ≥ 0.990 from Ct standard curve analysis.Slope from Ct standard curve analysis between -3.20 and -3.75.The +PAP reactions must show a ≥ 11 Ct difference from the “no-PAP” reactions.There must be at least three negative template controls with “undetermined” values.
DNaseDNase activity must be below the detectable limits of 100 pg DNase I equivalent as assayed using a fluorogenic substrate following a 1 hour incubation at 37°C with each kit component at 1X concentration.
RNaseRNase activity must be below the detectable limits of 1 pg RNase A equivalent as assayed using a fluorogenic substrate following a 1 hour incubation at 37°C with each kit component at 1X concentration
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