Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Journal of agricultural and food chemistry (2004-08-19)

Dawn M Chapman, John H Thorngate, Mark A Matthews, Jean-Xavier Guinard, Susan E Ebeler

ABSTRACT

A rapid and automated solid phase microextraction (SPME) stable isotope dilution gas chromatography/mass spectrometry (GC-MS) method for 2-methoxy-3-isobutylpyrazine (MIBP) quantification in red wine was developed. Wines with 30% (w/v) NaCl and 2-methoxy-(2)H(3)-3-isobutylpyrazine internal standard were sampled with a 2 cm divinylbenzene/carboxen/poly(dimethylsiloxane) SPME fiber for 30 min at 40 degrees C and analyzed by GC-MS. The method was used to measure MIBP concentrations in Cabernet Sauvignon wines that were produced from six winter pruning treatments over two vintages. MIBP concentrations were significantly negatively correlated with buds per vine. In addition, the MIBP concentration was directly related to sensory vegetal intensity ratings obtained by descriptive analysis.

MATERIALI

Numero di prodotto

Marchio

Descrizione del prodotto

57298-U

Supelco

Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, plain gray hub

57348-U

Supelco

Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 24 ga, Autosampler, pk of 3, notched gray hub

57328-U

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Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), 50/30µm DVB/CAR/PDMS, StableFlex (1cm), needle size 24 ga, Manual Holder, pk of 3, plain gray hub

57299-U

Supelco

Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 23 ga, Autosampler, pk of 3, notched gray hub

57329-U

Supelco

Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, plain gray hub

20358

Supelco

ORBO^™ 90 Carboxen^® 564 (20/45), 160/80 mg, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

20355

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ORBO^™ 78 HBr on Carboxen^® 564 specially cleaned (20/45), 400/200 mg, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea

25467

Supelco

Carboxen^®-1010 PLOT Capillary GC Column, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

10478-U

Supelco

Adsorbente Carboxen^®, matrix Carboxen^® 1000, 60-80 mesh, bottle of 10 g

57318

Supelco

Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, black hub

25461

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Colonna capillare per GC Carboxen^®-1006 PLOT, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57319

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Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, black hub

57295-U

Supelco

Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, light blue hub

24246

Supelco

Carboxen^®-1010 PLOT Capillary GC Column, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

57334-U

Supelco

Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 3, light blue hub

47528-U

Supelco

2-Isobutyl-3-methoxypyrazine solution, certified reference material

W313203

Sigma-Aldrich

2-Isobutyl-3-methoxypyrazine, ≥99%, FG

57335-U

Supelco

Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, light blue hub

23446-U

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ORBO^™-93 Sorbent Tube, ORBO tube I.D. × L 6 mm × 95 mm, Bed A 180 mg, Bed B 90 mg, 60/80 mesh, pkg of 25 ea

57343-U

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Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, black hub

297666

Sigma-Aldrich

2-Isobutyl-3-methoxypyrazine, 99%

24241-U

Supelco

Colonna capillare per GC Carboxen^®-1006 PLOT, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

28718-U

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Tenax^® TA / Carboxen^® 1018, glass TD tube, preconditioned, O.D. × L 1/4 in. × 3 1/2 in., Sealed with (Swagelok^® End-Fittings), pkg of 10 ea

57344-U

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10471

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Adsorbente Carboxen^®, matrix Carboxen^® 1003, 40-60 mesh, bottle of 10 g

57914-U

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10269

Supelco

Adsorbente Carboxen^®, matrix Carboxen^® 569, 20-45 mesh, bottle of 10 g

54041-U

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Carboxen^® 1000 Reversible SPE Tube, 200 mg/mL, pk 30