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Merck

C3041

Carbonate-Bicarbonate Buffer

capsule

Carbonate-Bicarbonate Buffer

Sinónimos:

Carbonate Buffer Solution

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50 CAPS

$251.000

100 CAPS

$445.000

$251.000


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UNSPSC Code:
12161700
NACRES:
NA.25

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form

capsule

color

colorless

pH

9.3-9.9

solubility

water, high purity: 1 capsule/100 mL

application(s)

diagnostic assay manufacturing

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Este artículo
88975T7408S5761
Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

300

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing
microbiology
sample preparation

application(s)

-

form

capsule

form

liquid

form

liquid

form

powder

pH

9.3-9.9

pH

9.5±0.2

pH

8.5±0.1

pH

6.8-7.4

solubility

water, high purity: 1 capsule/100 mL

solubility

-

solubility

-

solubility

water: soluble 100 mg/mL

General description

Bicarbonate buffer mostly have a pH close to 7.4. It is the major small intestine buffer.[1]

Application

Carbonate-Bicarbonate Buffer has been used for suspending the lyophilized human plasma sample for microtiter plate biofilm assay and flow cell biofilm assay.[2] It has also been used as a coating buffer for microtiter plates prior to enzyme-linked immunosorbent assay(ELISA).[3][4]
Prepared buffer is suitable for use in protein coating procedures on multiwell plates or plastic tubes.

Preparation Note

Empty the contents of one capsule in 100 mL of deionized water and dissolve. The contents of one capsule yields 100 ml of 0.05 M carbonate-bicarbonate buffer, pH 9.6 at 25 °C.

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Referencia del producto
Descripción
Precios

pictograms

Exclamation mark

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Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Clase de almacenamiento

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)
von Gersdorff J, et al.
Journal of Fish Diseases, 34(10), 749-755 (2011)
R M Jaafar et al.
Journal of fish diseases, 41(1), 117-123 (2017-07-15)
Teleosts are able to raise a protective immune response, comprising both innate and adaptive elements, against various pathogens. This is the basis for a widespread use of vaccines, administered as injection or immersion, in the aquaculture industry. It has been
L von Gersdorff Jørgensen et al.
Journal of fish diseases, 40(12), 1815-1821 (2017-05-27)
The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced
Mike Burrows et al.
BMC complementary and alternative medicine, 9, 49-49 (2009-12-02)
For almost 2000 years it has been recognized that aqueous extracts from pine cones possess medicinal properties beneficial for the treatment of a broad variety of diseases and conditions. In this report, the ability of an orally administered poly phenylpropanoid-polysaccharide
Ashfaq Ghumra et al.
PloS one, 6(1), e16414-e16414 (2011-02-10)
Rosetting is a Plasmodium falciparum virulence factor implicated in the pathogenesis of life-threatening malaria. Rosetting occurs when parasite-derived P. falciparum Erythrocyte Membrane Protein One (PfEMP1) on the surface of infected erythrocytes binds to human receptors on uninfected erythrocytes. PfEMP1 is

Protocolos

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

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Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. 純粋に溶解した場合、カプセル自体も溶解しますか。

    1 answer
    1. The capsules are not intended to be dissolved. After opening each capsule, the contents should be added to the appropriate volume of deionized water while stirring. The contents of one capsule is sufficient to prepare 100 mL of 0.05 M carbonate-bicarbonate buffer, pH 9.6. This buffer is comprised of 15 mM in sodium carbonate and 35 mM sodium bicarbonate.

      Helpful?

  3. If I make a solution and there are reagents left, how long can it be stored? What should I do with the storage conditions?

    1 answer
    1. Unfortunately, the long term stability of the prepared solution has not been determined. However, prepared solutions are expected to be quite stable. Similar Sodium Carbonate/Bicarbonate 20X solutions are assigned a 2 year shelf life. Provided that solutions are stored refrigerated and not contaminated, a comparable shelf life is expected. Solutions should be observed for any changes in color and turbidity. Please see the link below to review the prepared 20X Concentrate product options:
      https://www.sigmaaldrich.com/substance/sodiumbicarbonatesodiumcarbonateconcentrate1234598765

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  4. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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Reviews

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  1. Sanford, NC
    • Review 1
    • Votes 2
    5 out of 5 stars.

    Came as ordered, worked as expected

    The product was delivered on-time with helpful emails confirming my order and informing me of deliver times. The product worked as expected. Glad to be able to order the amounts that are needed and not have to order excess.

    Helpful?

  2. 1 Ratings-only review

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