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F3040

Monoclonal ANTI-FLAG^® M1 antibody

Name: Monoclonal ANTI-FLAG<SUP>®</SUP> M1 antibody
Brand: SIGMA
Price: 675000 CLP

clone M1, purified immunoglobulin, buffered aqueous solution

Sinónimos:

Anti-ddddk, Anti-dykddddk

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Talla/SKU	Disponibilidad	Precio
0.2 mg	Comprobar disponibilidad del carrito	$675.000
1 mg	Comprobar disponibilidad del carrito	$1.160.000
5 mg	Comprobar disponibilidad del carrito	$2.120.000

Acerca de este artículo

UNSPSC Code:

12352203

NACRES:

NA.32

MDL number:

MFCD00212941

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

M1, monoclonal

Application:

FLAG purification, WB

Citations:

139

$675.000

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Propiedades

biological source

(Bioreactor)

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M1, monoclonal

form

buffered aqueous solution

purified by

using Protein A

species reactivity

all

concentration

2-5 mg/mL

technique(s)

FLAG purification: suitable, western blot: 10 μg/mL

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Descripción

General description

The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

Monoclonal ANTI-FLAG^® M1 antibody is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.

Chromatin immunoprecipitation (1 paper)

Immunofluorescence (1 paper)

Learn more product details in our FLAG^® applications portal.

Biochem/physiol Actions

Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca²⁺-dependent; the complex dissociates in the absence of calcium ions.

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).

Preparation Note

Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Este artículo	F3165	F1804	A9594
Sigma-Aldrich F3040 Monoclonal ANTI-FLAG^® M1 antibody	Sigma-Aldrich F3165 ANTI-FLAG^® M2 monoclonal antibody produced in mouse	Sigma-Aldrich F1804 ANTI-FLAG^® M2 monoclonal antibody produced in mouse	Sigma-Aldrich A9594 ANTI-FLAG^® M2 monoclonal antibody produced in mouse
description clone M1, purified immunoglobulin, buffered aqueous solution	description clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)	description 1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)	description clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
clone M1, monoclonal	clone M2, monoclonal	clone M2, monoclonal	clone M2, monoclonal
biological source (Bioreactor)	biological source mouse	biological source mouse	biological source mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate CY3 conjugate
antibody form purified immunoglobulin	antibody form purified immunoglobulin (Purified IgG1 subclass)	antibody form affinity isolated antibody	antibody form purified immunoglobulin
species reactivity all	species reactivity all	species reactivity all	species reactivity all
technique(s) FLAG purification: suitable, western blot: 10 μg/mL	technique(s) western blot: 10 μg/mL (Protein A), FLAG purification: suitable	technique(s) FLAG purification: suitable	technique(s) direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone, FLAG purification: suitable

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