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Merck

L4524

Lipopolysaccharides from Escherichia coli O55:B5

purified by ion-exchange chromatography, TLR ligand tested

Sinónimos:

LPS

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5 MG

$390.000

10 MG

$679.000

25 MG

$1.370.000

$390.000

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Número CAS:
93572-42-0
EC Number:
297-473-0
MDL number:
MFCD00164401
UNSPSC Code:
12352201
NACRES:
NA.25

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biological source

Escherichia coli (O55:B5)

Quality Level

200

form

lyophilized powder

purified by

ion-exchange chromatography

impurities

<1% Protein
<1% RNA

color

white to yellow cast

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

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1 of 4

Este artículo
L4005L2762L5418
Lipopolysaccharides from Escherichia coli O55:B5 purified by ion-exchange chromatography, TLR ligand tested

Sigma-Aldrich

L4524

Lipopolysaccharides from Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O55:B5 purified by trichloroacetic acid extraction

Sigma-Aldrich

L4005

Lipopolysaccharides from Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O26:B6 purified by gel-filtration chromatography

Sigma-Aldrich

L2762

Lipopolysaccharides from Escherichia coli O26:B6

Lipopolysaccharides from Escherichia coli O55:B5 Ready Made solution, 1&#160;mg/mL

Sigma-Aldrich

L5418

Lipopolysaccharides from Escherichia coli O55:B5

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O26:B6)

biological source

Escherichia coli (O55:B5)

solubility

water: soluble

solubility

water: soluble

solubility

water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow

solubility

water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

aqueous solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

impurities

<1% Protein, <1% RNA

impurities

1-10% Protein (Lowry)

impurities

<5% Protein

impurities

-

General description

This product is extracted from E. coli serotype O55:B5 and purified by ion exchange. The source strain is CDC 1644-70. The LPS O55:B5 has been used to stimulate human peritoneal macrophages at 1 ng/mL and to stimulate equine peritoneal macrophages at 1-100 ng/mL.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

related product

Referencia del producto
Descripción
Precios

L4524

Lipopolysaccharides from Escherichia coli O55:B5, purified by ion-exchange chromatography, TLR ligand tested

L4005

Lipopolysaccharides from Escherichia coli O55:B5, purified by trichloroacetic acid extraction

L6529

Lipopolysaccharides from Escherichia coli O55:B5, γ-irradiated, BioXtra, suitable for cell culture

L2637

Lipopolysaccharides from Escherichia coli O55:B5, purified by gel-filtration chromatography

L2880

Lipopolysaccharides from Escherichia coli O55:B5, purified by phenol extraction

F8666

Lipopolysaccharides from Escherichia coli O55:B5, FITC conjugate

L5418

Lipopolysaccharides from Escherichia coli O55:B5, Ready Made solution, 1 mg/mL

pictograms

Skull and crossbones
GHS06

signalword

Danger

hcodes

H300

Hazard Classifications

Acute Tox. 2 Oral

Clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000476847
0000476847
0000476849
0000476848
0000476849

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Visite la Librería de documentos

Yoshitaka Shirasaki et al.
Scientific reports, 4, 4736-4736 (2014-04-23)
Protein secretion, a key intercellular event for transducing cellular signals, is thought to be strictly regulated. However, secretion dynamics at the single-cell level have not yet been clarified because intercellular heterogeneity results in an averaging response from the bulk cell
Arij Faksh et al.
Pediatric research, 79(3), 391-400 (2015-11-06)
Antenatal inflammation and preterm birth are associated with the development of airway diseases such as wheezing and asthma. Utilizing a newborn mouse model, we assessed the effects of maternal inflammation and postnatal hyperoxia on the neonatal airway. Pregnant C57/Bl6 dams
Siroon Bekkering et al.
Clinical science (London, England : 1979), 133(10), 1185-1196 (2019-05-16)
Atherosclerosis is a chronic inflammatory disease that has its origins in early life. Postnatal inflammation exacerbates atherosclerosis, but the possible effect of intrauterine inflammation is largely unexplored. Exposure to inflammation in utero is common, especially in infants born preterm, who
Laura B Fanning et al.
PloS one, 8(2), e57007-e57007 (2013-02-23)
Leukocyte Immunoglobulin-like Receptor B4 (LILRB4) null mice have an exacerbated T helper cell type 2 (Th2) immune response and pulmonary inflammation compared with Lilrb4(+/+) animals when sensitized intranasally with ovalbumin (OVA) and low-dose lipopolysaccharide (LPS) followed by challenge with OVA.
Li Liu et al.
Journal of cellular and molecular medicine, 19(12), 2728-2740 (2015-08-21)
It remains unclear whether and how cardiomyocytes contribute to the inflammation in chronic heart failure (CHF). We recently reviewed the capacity of cardiomyocytes to initiate inflammation, by means of expressing certain immune receptors such as toll-like receptors (TLRs) that respond
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Contenido relacionado

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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