Yes, this product is suitable for coating culture surfaces such as plates, flasks, slides, and cover slips. After the coated surface is completely dry store in a sealed container or ziplock for up to one year. Do not use if coated surface becomes yellow or cloudy. Please see the link below to review the product datasheet for a similar item, P4707. This information may also be applied to P4832:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/244/731/p4707pis.pdf
P4832
Poly-ʟ-Lysine Hydrobromide
synthetic, mol wt 150,000-300,000, solution, 0.01%, suitable for cell culture
Poly-L-lysine solution
Sinónimos:
PDL Solution
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Permítanos ayudarleNombre del producto
Poli-L-lisina solution, mol wt 150,000-300,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture
biological source
synthetic
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
solution
mol wt
150,000-300,000
packaging
pkg of 50 mL
concentration
0.01%
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
solubility
water: soluble
shipped in
ambient
storage temp.
2-8°C
SMILES string
N([C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)C(=O)[C@@H](N)CCCCN
InChI
1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
InChI key
WBSCNDJQPKSPII-KKUMJFAQSA-N
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Este artículo | P4707 | P9155 | P6282 |
|---|---|---|---|
| biological source synthetic | biological source synthetic | biological source synthetic (organic) | biological source synthetic (organic) |
| sterility sterile-filtered | sterility sterile-filtered | sterility γ-irradiated | sterility γ-irradiated |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| concentration 0.01% | concentration 0.01% | concentration - | concentration - |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| form solution | form liquid | form lyophilized powder | form lyophilized powder |
General description
L-lysine is a homopolypeptide having two enantiomeric forms, L and D lysine. Through its three reactive groups (the carboxylic acid and the α and ε amines), L-lysine has the capacity to generate various branched polymeric structures, including dendrimers, branched polylysine, and hyperbranched polylysine (PLL). [1] Poly-L-lysine is a polymer made up of positively charged amino acid residues, with each lysine residue typically containing one HBr.
Application
Los polímeros de poli-L-lisina pueden utilizarse en la promoción de la adhesión celular a los sustratos sólidos, la conjugación al metotrexato para incrementar el transporte de medicamentos, la microencapsulación de islote, la tecnología de microencapsulación celular, recubrimiento de portaobjetos de vidrio de micromatrices y preparaciones cromosómicas. La poli-L-lisina de menor peso molecular (30 000-70 000) es menos viscosa en disolución, pero las versiones de mayor peso molecular proporcionan mas sitios de unión por molécula.
Poly-L-lysine has been used:
- In coating 96-well plates for cell attachment in testing respiratory burst activity.[2]
- In coating glass cover slips for immunofluorescent labeling of intracellular structures.[3]
- For pretreating 6-well plate in myosatellite cell isolation protocol.[4]
- In coating coverslips for cell adhesion in E14 neuron culture to study possible mechanism of action of circRNA_01477 in neurons.[5]
Biochem/physiol Actions
La poli-L-lisina es un factor de unión inespecífica a las células útil en la promoción de la adhesión celular a los sustratos sólidos intensificando la interacción electrostática entre iones con carga negativa de la membrana celular y la superficie del cultivo. Cuando se absorbe a la superficie del cultivo celular, la poli-L-lisina funciona aumentando el número de sitios con carga positiva disponibles para la unión a la célula. Con las células que pueden digerir la poli-L-lisina, debe utilizarse la poli-D-lisina como factor de fijación.
Poly‐l‐lysine (PLL) acts as polycationic polymers that have been extensively used to carry drugs, genes etc. across the cell membrane. [6] Poly-L-lysine (PLL) has the capability to engage in the formation of polyelectrolyte complexes (PECs) by interacting with anionic entities such as polyanions, nucleic acid chains and some drugs.[6]
Preparation Note
This product is supplied as a 0.01% sterile filtered solution in water, using a molecular weight of 150 - 300 kDa. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.
Other Notes
La poli-L-lisina es una polímero de aminoácido con carga positiva con aproximadamente un HBr por residuo de lisina. El hidrobromuro permite a la poli-L-lisina estar en una forma cristalina hidrosoluble. Puede encontrarse una pequeña cantidad del producto en la estructura beta porque el HBr interfiere en el enlace de hidrógeno entre el grupo amino y los grupos carboxilo o los grupos que contienen N u O.
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Clase de almacenamiento
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Modulating the poly-L-lysine structure through the control of the protonation-deprotonation state of L-lysine
Luigi Stagi, et al.
Scientific Reports, 12(1), 19719-19719 (2022)
Effects of sex steroids on indices of protein turnover in rainbow trout (Oncorhynchus mykiss) white muscle
Cleveland BM and Weber GM
General and Comparative Endocrinology, 174(2), 132-142 (2011)
Polylysine for skin regeneration: A review of recent advances and future perspectives
Zarrintaj P, et al.
Bioengineering & translational medicine, 7(1), e10261-e10261 (2020)
Caitlin N Spaulding et al.
eLife, 7 (2018-01-19)
Uropathogenic E. coli (UPEC), which cause urinary tract infections (UTI), utilize type 1 pili, a chaperone usher pathway (CUP) pilus, to cause UTI and colonize the gut. The pilus rod, comprised of repeating FimA subunits, provides a structural scaffold for
Modulating the poly-L-lysine structure through the control of the protonation?deprotonation state of L-lysine
Stagi L, et al.
Scientific Reports (2022)
Artículos
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
Protocolos
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.
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Can I coat a tissue culture plate and store it ? What is the best way to store for future use?
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