62971

Lysozyme from chicken egg white

powder (crystalline), 70000-140000 U/mg

• Synonyma: Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• Číslo CAS: 12650-88-3
• UNSPSC Code: 12352204
• NACRES: NA.22
• EC Number: 235-747-3
• MDL number: MFCD00131557
• Číslo EC: 3.2.1.17 (BRENDA, IUBMB)
• Specific activity: 70000-140000 U/mg
• Biological source: chicken egg white

Vlastnosti

• biological source: chicken egg white
• Quality Segment: 100
• form: powder (crystalline)
• specific activity: 70000-140000 U/mg
• mol wt: single-chain 14.3 kDa, M_r ~14600
• technique(s): cell based assay: suitable
• input: bacteria and yeast sample(s)
• suitability: suitable for cell lysis
• UniProt accession no.: P00698
• application(s): cell analysis
• storage temp.: 2-8°C
• Gene Information: chicken ... LYZ(396218)

Popis

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.

Other Notes

1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus, ATCC 4698, as substrate)

Bezpečnostní informace

• Piktogramy: GHS08
• Výstražné slovo: Danger
• Kódy nebezpečí: H334
• Bezpečnostní pokyny: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Skladovací třída: 11 - Combustible Solids
• Co se děje?: WGK 3
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• PPE (osobní ochranné prostředky): Eyeshields, Gloves, type N95 (US)