NA0300

GenElute^™ HP Plasmid Maxiprep Kit

sufficient for 10 purifications

• Synonyma: GenElute^™ HP Plasmid Maxiprep Kit
• NACRES: NA.52
• UNSPSC Code: 41105501

Vlastnosti

• usage: sufficient for 10 purifications
• Quality Segment: 200
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Sustainability features
• technique(s): DNA purification: suitable
• test parameters: sample volume: 50 mL bacterial culture
• storage temp.: 15-25°C

Popis

General description

Click here to view the kit protocol

The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

GenElute^™ HP Plasmid Maxiprep Kit has been used in plasmid DNA extraction of SF91 or MIEV vector, apolipoprotein A-V mutant constructs, lentiviral vector pLenti6/R4R2/V5-DEST,metastasis-associated protein 1 (MTA1) and adipokinetic hormone receptor

Features and Benefits

• From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
• Up to 1.2 mg of high-copy plasmid DNA
• Flexibility of a vacuum or spin format
• Contains fewer plastic components than other high speed kits, reducing the amount of waste
• No phenol/chloroform extraction or alcohol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Součásti soupravy, které jsou dostupné také samostatně

• L1912

  Lysis Solution

  Bezpečností list
• W4639

  Wash Solution 2

  Bezpečností list
• R6148

  RNase A solution

  Bezpečností list
• R1149

  Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )

  Bezpečností list
• C2112

  Column Preparation Solution

  Bezpečností list
• W0263

  Wash Solution 1

  Bezpečností list

Bezpečnostní informace

• Piktogramy: GHS02,GHS08,GHS05,GHS07
• Výstražné slovo: Danger
• Bezpečnostní pokyny: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Cílové orgány: Central nervous system
• Skladovací třída: 3 - Flammable liquids
• Bod vzplanutí (°F): 77.0 °F - closed cup
• Bod vzplanutí (°C): 25 °C - closed cup
• Co se děje?: WGK 3
• Kódy nebezpečí: H226,H290,H302 + H332,H314,H334,H336
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3