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PLX15

GenElute™ Plasmid Maxiprep Kit

sufficient for 15 purifications

Funkce v oblasti udržitelnosti

Synonyma:

Gen Elute, GenElute™ Plasmid Kit

Přihlásit pro zobrazení organizačních a smluvních cen.

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Vám/SKUDostupnostCena
1 kit
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
20 200,00 Kč

O této položce

NACRES:
NA.52
UNSPSC Code:
41105501

20 200,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
​
Technický servis
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usage

sufficient for 15 purifications

Quality Segment

sustainability

Sustainability features

technique(s)

DNA purification: suitable

test parameters

: ~45 min kit run time, sample volume: 25-200 mL bacterial culture

greener alternative category

storage temp.

15-25°C

General description

Click here to view the kit protocol
The GenElute Plasmid Maxiprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 1.2 mg of plasmid DNA can be recovered from 25-200 ml of Luria Broth (LB) in about 45 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.

This product aligns with one or more of the 12 Principles of Green ChemistryThis product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The recovered plasmid DNA is predominantly in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination detected by agarose gel electrophoresis. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, and transfection.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Tato položka
NA0410NA0310NA0300
GenElute™ Plasmid Maxiprep Kit sufficient for 15 purifications

Sigma-Aldrich

PLX15

GenElute™ Plasmid Maxiprep Kit

GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit sufficient for 25 preparations

Sigma-Aldrich

NA0410

GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit

GenElute™ HP Plasmid Maxiprep Kit sufficient for 25 purifications

Sigma-Aldrich

NA0310

GenElute™ HP Plasmid Maxiprep Kit

GenElute™ HP Plasmid Maxiprep Kit sufficient for 10 purifications

Sigma-Aldrich

NA0300

GenElute™ HP Plasmid Maxiprep Kit

description

sufficient for 15 purifications

description

sufficient for 25 preparations

description

sufficient for 25 purifications

description

sufficient for 10 purifications

usage

sufficient for 15 purifications

usage

sufficient for 25 preparations

usage

sufficient for 25 purifications

usage

sufficient for 10 purifications

technique(s)

DNA purification: suitable

technique(s)

DNA extraction: suitable

technique(s)

DNA purification: suitable

technique(s)

DNA purification: suitable

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

sustainability

Sustainability features

sustainability

Sustainability features

sustainability

Sustainability features

sustainability

Sustainability features

test parameters

sample volume: 25-200 mL bacterial culture, : ~45 min kit run time

test parameters

sample volume: 150 mL bacterial culture

test parameters

sample volume: 150 mL bacterial culture

test parameters

sample volume: 50 mL bacterial culture

Výstražné slovo

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Cílové orgány

Central nervous system

Skladovací třída

10 - Combustible liquids

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

WGK

WGK 3

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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

  1. Hi: Does this plasmid prep involve alkali lysis?

    1 answer

    1. Yes, the recombinant E. coli culture is subjected to a modified alkaline-SDS lysis procedure prior to the adsorption of the DNA onto silica.

      Please see the introduction to the product data sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/213/607/plx15bul.pdf

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