A midiprep or maxiprep requires only 30 minutes to complete.
NA0310
GenElute™ HP Plasmid Maxiprep Kit
sufficient for 25 purifications
Funkce v oblasti udržitelnosti
Synonyma:
GenElute™ HP Plasmid Maxiprep Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit
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Vybrat velikost
Změnit zobrazení
| Vám/SKU | Dostupnost | Cena |
|---|---|---|
1 kit | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 25 500,00 Kč |
O této položce
NACRES:
NA.52
UNSPSC Code:
41105501
25 500,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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sufficient for 25 purifications
Quality Segment
sustainability
Sustainability features
technique(s)
DNA purification: suitable
test parameters
sample volume: 150 mL bacterial culture
storage temp.
15-25°C
General description
The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
GenElute™ HP Plasmid Maxiprep Kit has been used in the isolation of plasmids.
Features and Benefits
- From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
- Up to 1.2 mg of high-copy plasmid DNA
- Flexibility of a vacuum or spin format
- Contains fewer plastic components than other high speed kits, reducing the amount of waste
- No phenol/chloroform extraction or alcohol precipitation required
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
1 of 1
Tato položka | |||
|---|---|---|---|
| description sufficient for 25 purifications | description sufficient for 15 purifications | description sufficient for 10 preparations | description sufficient for 25 preparations |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA extraction: suitable | technique(s) DNA extraction: suitable |
| usage sufficient for 25 purifications | usage sufficient for 15 purifications | usage sufficient for 10 preparations | usage sufficient for 25 preparations |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Součásti soupravy, které jsou dostupné také samostatně
Č. produktu
Popis
Bezpečností list & Stanovení ceny
Výstražné slovo
Danger
Cílové orgány
Central nervous system
Skladovací třída
3 - Flammable liquids
Bod vzplanutí (°F)
77.0 °F - closed cup
Bod vzplanutí (°C)
25 °C - closed cup
Co se děje?
WGK 3
Kódy nebezpečí
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
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How long does it take to isolate plasmid using the vacuum format?
1 answer-
Helpful?
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Which format gives a better yield, vacuum or spin?
1 answer-
There is no difference in plasmid recovery when using either format.
Helpful?
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Do the binding columns fit on other manufacturers’ manifolds?
1 answer-
Yes. The columns fit on all standard vacuum manifolds.
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Is plasmid DNA isolated with this kit suitable for use in transfection?
1 answer-
Yes. However, if you are currently required to use an endofree plasmid purification kit, we recommend using the GenElute HP Endotoxin-free Kit.
Helpful?
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How do the GenElute HP Plasmid Kits differ from other plasmid purification systems?
1 answer-
The GenElute HP Plasmid Kits are much faster than other commercially available kits. A filter syringe is used for preparing the cleared lysate, which replaces a lengthy centrifugation step. These kits also offer the option of using a vacuum or spin purification format rather than a gravity drip to bind, wash and elute the plasmid. In addition, samples are eluted in Tris buffer so there are no alcohol precipitations required.
Helpful?
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What is the expected yield of plasmid DNA that you can isolate from these kits?
1 answer-
Yields can reach as high as 350 mg for a midiprep and 1.2 mg for a maxiprep. As with any protocol, lower yields can be expected with low copy plasmids.
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