Product A9594, Monoclonal ANTI-FLAG® M2-Cy3™ antibody produced in mice, is mouse IgG1. Theoretically, it will be recognized by the anti-mouse IgG secondary antibody. However, Product A9594 is already conjugated with Cy3 so a secondary antibody is not needed for detection.
A9594
Monoclonal ANTI-FLAG® M2-Cy3 antibody, Mouse monoclonal
clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
Synonyma:
Monoclonal ANTI-FLAG® M2, Anti-ddddk, Anti-dykddddk
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Vybrat velikost
Změnit zobrazení
| Vám/SKU | Dostupnost | Cena |
|---|---|---|
0.2 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 16 200,00 Kč |
1 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 36 700,00 Kč |
5 x 1 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 56 300,00 Kč |
O této položce
NACRES:
NA.32
UNSPSC Code:
41106514
Conjugate:
CY3 conjugate
Clone:
M2, monoclonal
Application:
FLAG purification, IF (d)
Citations:
42
16 200,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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mouse
Quality Segment
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
species reactivity
all
concentration
~1 mg/mL
technique(s)
FLAG purification: suitable, direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
General description
Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
Physical form
Solution in phosphate buffered saline plus 1% BSA and preservative
Preparation Note
Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.
Other Notes
Suggested concentration of 1-10 mg/ml for immunocytochemistry.
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
Tato položka | |||
|---|---|---|---|
| description clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate) | description clone M2, purified immunoglobulin, buffered aqueous glycerol solution | description 1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4) | description Clone M2, purified from hybridoma cell culture in bioreactor |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone M2, monoclonal | clone M2, monoclonal | clone M2, monoclonal | clone M2, monoclonal |
| species reactivity all | species reactivity all | species reactivity all | species reactivity - |
| conjugate CY3 conjugate | conjugate biotin conjugate | conjugate unconjugated | conjugate - |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form purified immunoglobulin (purified IgG1 subclass) |
| form buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate) | form buffered aqueous glycerol solution | form buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4) | form - |
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Skladovací třída
10 - Combustible liquids
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
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A9594（FLAG M2 Cy3標識）の抗体は抗マウスIgGの2次抗体に認識されますか。
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