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F3040

Monoclonal ANTI-FLAG^® M1 antibody

Name: Monoclonal ANTI-FLAG<SUP>®</SUP> M1 antibody
Brand: SIGMA
Price: 16000 CZK

clone M1, purified immunoglobulin, buffered aqueous solution

Synonyma:

Anti-ddddk, Anti-dykddddk

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Vám/SKU	Dostupnost	Cena
0.2 mg	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	16 000,00 Kč
1 mg	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	27 200,00 Kč
5 mg	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	50 000,00 Kč

O této položce

UNSPSC Code:

12352203

NACRES:

NA.32

MDL number:

MFCD00212941

Conjugate:

unconjugated

Clone:

M1, monoclonal

Application:

FLAG purification, WB

Citations:

139

16 000,00 Kč

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Vlastnosti

biological source

(Bioreactor)

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M1, monoclonal

form

buffered aqueous solution

purified by

using Protein A

species reactivity

all

concentration

2-5 mg/mL

technique(s)

FLAG purification: suitable, western blot: 10 μg/mL

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Popis

General description

The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

Monoclonal ANTI-FLAG^® M1 antibody is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.

Chromatin immunoprecipitation (1 paper)

Immunofluorescence (1 paper)

Learn more product details in our FLAG^® applications portal.

Biochem/physiol Actions

Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca²⁺-dependent; the complex dissociates in the absence of calcium ions.

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).

Preparation Note

Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Tato položka	F3165	A9594	F9291
Sigma-Aldrich F3040 Monoclonal ANTI-FLAG^® M1 antibody	Sigma-Aldrich F3165 ANTI-FLAG^® M2 antibody, Mouse monoclonal	Sigma-Aldrich A9594 Monoclonal ANTI-FLAG^® M2-Cy3 antibody, Mouse monoclonal	Sigma-Aldrich F9291 ANTI-FLAG^® BioM2 antibody, Mouse monoclonal
description clone M1, purified immunoglobulin, buffered aqueous solution	description clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)	description clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)	description clone M2, purified immunoglobulin, buffered aqueous glycerol solution
conjugate unconjugated	conjugate unconjugated	conjugate CY3 conjugate	conjugate biotin conjugate
biological source (Bioreactor)	biological source mouse	biological source mouse	biological source mouse
clone M1, monoclonal	clone M2, monoclonal	clone M2, monoclonal	clone M2, monoclonal
species reactivity all	species reactivity all	species reactivity all	species reactivity all
antibody form purified immunoglobulin	antibody form purified immunoglobulin (Purified IgG1 subclass)	antibody form purified immunoglobulin	antibody form purified immunoglobulin
technique(s) western blot: 10 μg/mL, FLAG purification: suitable	technique(s) western blot: 10 μg/mL (Protein A), FLAG purification: suitable	technique(s) FLAG purification: suitable, direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone	technique(s) FLAG purification: suitable, dot blot: suitable (chemiluminescent detection)

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Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

Skladovací třída

12 - Non Combustible Liquids

Co se děje?

WGK 2

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