This is determined by a spectral assay. The detailed assay procedure (operating procedure #11252) may be requested from Sigma St. Louis Technical Service (techserv@sial.com).
C2867
Cytochrome c from equine heart
BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture
Synonyma:
CYC, CytC, Cytochrome c from horse heart
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Vybrat velikost
Změnit zobrazení
| Vám/SKU | Dostupnost | Cena |
|---|---|---|
10 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 5 720,00 Kč |
50 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 20 800,00 Kč |
O této položce
Form:
powder
Assay:
≥99% (SDS-PAGE)
Biological source:
horse heart
Mol wt:
12,384
5 720,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Dovolte nám, abychom vám pomohlibiological source
horse heart
Quality Segment
product line
BioUltra
assay
≥99% (SDS-PAGE)
form
powder
mol wt
12,384
storage condition
(Tightly closed Dry)
technique(s)
cell culture | mammalian: suitable
solubility
water: 10 mg/mL, dark red-brown
UniProt accession no.
storage temp.
−20°C
Gene Information
horse ... CYCS(100053958)
General description
Application
Cytochrome c from equine heart has been used to prepare a stock solution for synthesizing and calibrating biotinylated cytochrome c. It has also been used as a component of the reaction buffer to stain electron transport chain (ETC) complex IV and determine its activity using blue-native gel electrophoresis.
The specific sites and extent of oxidation in horse cytochrome c treated with H2O2 and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell.
Biochem/physiol Actions
Cytochrome C (Cyt C) acts as a potent signaling molecule of apoptosis by activating caspase 3, a death protease. It plays a role in heme-binding with the help of cytochrome c heme lyase which facilitates its release into the mitochondrial intermembrane space. Cyt C also exhibits lipid-binding by interacting with cardiolipin, a phospholipid that initiates protein unfolding and activates the peroxidase function in Cyt C.
Cytochrome c is primarily known as an electron-carrying mitochondrial protein. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. It is generally regarded as a universal catalyst of respiration, forming an essential electron-bridge between the respirable substrates and oxygen
Preparation Note
A further purification of C2506
Prepared using TCA.
Other Notes
View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.
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Tato položka | |||
|---|---|---|---|
| description BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture | description ≥95% based on Mol. Wt. 12,384 basis | description ≥95% (SDS-PAGE) | description BioReagent, suitable for GFC marker |
| assay ≥99% (SDS-PAGE) | assay ≥95% based on Mol. Wt. 12,384 basis, ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay - |
| biological source horse heart | biological source horse heart | biological source horse heart | biological source - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell based assay: suitable | technique(s) - |
| mol wt 12,384 | mol wt 12,384 | mol wt 12,384 | mol wt ~12,400 |
| form powder | form powder | form powder | form powder |
| solubility water: 10 mg/mL, dark red-brown | solubility - | solubility H2O: soluble 10 mg/mL | solubility - |
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Skladovací třída
11 - Combustible Solids
WGK
WGK 3
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
PPE (osobní ochranné prostředky)
Eyeshields, Gloves, type N95 (US)
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How do I determine how much of Product C2867, Cytochrome c, is in the oxidized form, and how much is reduced?
1 answer-
Helpful?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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I see that you list several horse heart cytochrome c products. What are the differences, and which one should I choose?
1 answer-
Product No. C2506 is prepared with trichloroacetic acid (TCA), whereas C7752 is prepared with the milder acetic acid. The TCA method may reduce the amount of superoxide dismutase (SOD) present, but tends to cause dimerization or acid-modified structures of cytochrome c. In contrast, acetic acid preparations may have slightly higher amounts of SOD, but a lower proportion of dimeric cytochrome c. So C2506 would be recommended for enzyme activity studies that require low SOD, whereas C7752 is recommended for structural studies or binding studies, or for enzyme activity studies where possible SOD contamination is not a problem. Product No. C2867 is prepared from C2506; it is a BioUtra grade product, greater than or equal to 99% pure as determined by SDS-PAGE. It is recommended when an ultra-high purity is required. Product No. C3256 is also prepared from C7752; it is isolated by ion-exchange chromatography. This fraction shows a high rate of ascorbic acid oxidation, but low activity in a cytochrome oxidase system. Product No. C7150 is also prepared from C7752; it is suitable for use as a gel filtration molecular weight marker.
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How should I store solutions of Product C2867, Cytochrome c?
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Our general recommendations are as follows: Store at -20 °C (freezer) for up to six months. Store at 2-8 °C (refrigerator) for up to two weeks. Store at 20-25 °C (room temperature) for up to three days. According to J. Biol. Chem, 237, 2161-2174 (1962), cytochrome c is stable to boiling, and over an unusually wide range of pH values. However, it denatures readily at extreme pH (low or high), and in high concentrations of organic solvents, such as ethanol or acetone.
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Is Product C2867, Cytochrome c, predominantly in the oxidized form or the reduced form?
1 answer-
This product is predominantly (>95%) in the oxidized form.
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