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CS1070

Transglutaminase Assay Kit

sufficient for assays in two 96-well plates

Akční nabídka

Synonyma:

TGases Assay Kit

Přihlásit pro zobrazení organizačních a smluvních cen.

Utratit 10 750 Kč – Ušetřit 5 %; Utratit 15 250 Kč – Ušetřit 10 %; Utratit 28 000 Kč – Ušetřit 15 %*

Přihlásit se pro ověření způsobilosti. Obchod s produkty

Vybrat velikost

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Vám/SKUDostupnostCena
1 kit
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
25 600,00 Kč

O této položce

NACRES:
NA.28
UNSPSC Code:
12161503

25 600,00 Kč

Ušetřete až 15 %


Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.

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Quality Segment

shipped in

dry ice

storage temp.

−20°C

General description

The Transglutaminase Assay Kit is designed for the detection of transglutaminase activity in biological samples. The kit can also be used for screening of transglutaminase inhibitors/activators.

Application

The assay is based on transglutaminase catalysis of a covalent bond formation between the free amine group of Poly L-Lysine, which is covalently attached to the plate surface, and the γ-carboxamide group of biotin-TVQQEL-OH substrate present in the assay buffer. This reaction immobilizes biotin to the plate surface. The amount of immobilized biotin is proportional to the amount of active transglutaminase in the sample. The amount of immobilized biotin is determined using streptavidin-peroxidase and TMB substrate.

Biochem/physiol Actions

Transglutaminases (TGases) are a widely distributed and unique group of calcium dependent enzymes that catalyze the post-translational modification of proteins by the formation of isopeptide bonds. This occurs either through protein cross-linking via formation of γ-glutamyl-ε-lysine bonds or through incorporation of primary amines at selected peptide-bound glutamine residues. The cross-linked products, often of high molecular mass, are highly resistant to mechanical challenge and proteolytic degradation, and their accumulation is found in a number of tissues and processes, where such properties are important including skin, hair, blood clotting, and wound healing. Deregulation of the enzyme activity contributes to a number of human diseases.

Other Notes

The detection limit is 0.003 mU/assay or 0.03mU/ml of Transglutaminase.

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Tato položka
WH0007052M10RAB1741AV47471
description

sufficient for assays in two 96-well plates

description

clone 2F4, purified immunoglobulin, buffered aqueous solution

description

for serum, plasma and cell culture supernatants

description

affinity isolated antibody

Gene Information

human ... TGM1(7051), TGM2(7052), TGM3(7053), TGM4(7047), TGM5(9333), TGM6(343641), TGM7(116179)

Gene Information

human ... TGM2(7052)

Gene Information

human ... TGM2(7052)

Gene Information

human ... TGM2(7052)

Quality Level

200

Quality Level

100

Quality Level

-

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice


Součásti soupravy, které jsou dostupné také samostatně

Č. produktu
Popis
Bezpečností list & Stanovení ceny

  • Transglutaminase from guinea pig liver, lyophilized powder, ≥1.5 units/mg protein 2 ea
    Bezpečností list

  • 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, peroxidase substrate
    Bezpečností list

  • Streptavidin−Peroxidase from Streptomyces avidinii, lyophilized powder 100 μg
    Bezpečností list

Piktogramy

Health hazardCorrosion

Výstražné slovo

Danger

Co se děje?

WGK 3

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B

Skladovací třída

8A - Combustible corrosive hazardous materials



Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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