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D4293

3,3′-Diaminobenzidine

SIGMAFAST™, chromogenic, tablet

Synonyma:

DAB tablets

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Vám/SKUDostupnostCena
5 sets
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1 300,00 Kč
50 sets
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
6 300,00 Kč
5 x 50 set
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
23 500,00 Kč

O této položce

Číslo CAS:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:

1 300,00 Kč


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Název produktu

SIGMAFAST™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 5 mL

Quality Segment

form

tablet

storage temp.

−20°C

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

Application

SIGMAFAST™ 3,3′-Diaminobenzidine tablets has been used as a chromogen for visualization in immunohistochemistry of gonad tissue, thymus, dental chronic periapical lesions and gall bladder sections.
Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Preparation Note

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Tato položka
D4418D8001N2770
description

tablet, To prepare 5 mL

description

tablet, To prepare 15 mL

description

97% (HPLC)

description

tablet, To prepare 20 mL

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

tablet

form

tablet

form

powder

form

tablet

storage temp.

−20°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C


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signalword

Danger

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Skladovací třída

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3



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Lot/Batch Number

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Questions

1–2 of 2 Questions  
  1. Hello, your product D4293, SIGMAFAST™ 3,3′-Diaminobenzidine tablets, can be used for how many months after dissolving in 5ml of distilled water?

    1 answer
    1. This product is a set of two tablets - one DAB tablet and one Urea Hydrogen Peroxide tablet. When prepared according to instructions, the solution should be used within one hour for best results.

      Helpful?

  2. Hello, Can https://www.sigmaaldrich.com/US/en/product/sigma/d4293?icid=sharepdp-clipboard-copy-productdetailpage this product be used to develop western blots? Thank you, Regards Vanda

    1 answer
    1. D4293, SIGMAFAST™ 3,3′-Diaminobenzidine tablets, can be used for WB on PVDF membranes, please see attached reference paper stating below: "The PVDF was then collected and rinsed 3 times for 5–10 min each wash with TBS. The blot was then developed appropriately with 3',3-Diaminobenzidine tablets for developing peroxidase (D4293 Sigma-Aldrich) and BCIP/NBT for alkaline phosphatase (B5655 Sigma-Aldrich) and the blot was imaged."Scientific Neurogenic marker expression in differentiating human adipose derived adult mesenchymal stem cells Neus Gomila Pelegri1,2, Bruce K. Milthorpe1 , Catherine A. Gorrie2 , Jerran Santos1. Stem Cell Investig 2023;10:7

      Helpful?

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