Přejít k obsahu
Merck

Přeskočit na

D8130

5,5′-Dithiobis(2-nitrobenzoic acid)

≥98%, BioReagent, suitable for determination of sulfhydryl groups

Synonyma:

3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent

Přihlásit pro zobrazení organizačních a smluvních cen.

Vybrat velikost

Změnit zobrazení
Vám/SKUDostupnostCena
500 mg
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
1 030,00 Kč
1 g
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
1 870,00 Kč
5 g
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
4 680,00 Kč
10 g
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
7 980,00 Kč
25 g
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
16 400,00 Kč
100 g
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
64 700,00 Kč
54 995,00 Kč

O této položce

Lineární vzorec:
[-SC6H3(NO2)CO2H]2
Číslo CAS:
Molekulová hmotnost:
396.35
UNSPSC Code:
12352106
NACRES:
NA.26
PubChem Substance ID:
EC Number:
200-714-4
Beilstein/REAXYS Number:
1896821
MDL number:
Assay:
≥98%
Form:
powder

1 030,00 Kč


Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.

​
Požádejte o zakázkovou objednávku
Technický servis
Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.
Dovolte nám, abychom vám pomohli


product line

BioReagent

Quality Segment

assay

≥98%

form

powder

mp

240-245 °C (dec.) (lit.)

suitability

suitable for determination of sulfhydryl groups

SMILES string

OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

General description

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.

Application

Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues. It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues.

Porovnat podobné položky

Zobrazit úplné porovnání

Zobrazit rozdíly

1 of 1

Tato položka
D218200322123158194
description

≥98%, BioReagent, suitable for determination of sulfhydryl groups

description

ReagentPlus®, 99%

description

Sulfhydryl reagent used to characterize reactive SH groups.

description

96%

assay

≥98%

assay

99%

assay

≥98% (titration)

assay

96%

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

form

powder

form

powder

form

solid

form

solid

mp

240-245 °C (dec.) (lit.)

mp

240-245 °C (dec.) (lit.)

mp

-

mp

155-157 °C (lit.)

suitability

suitable for determination of sulfhydryl groups

suitability

-

suitability

-

suitability

-

product line

BioReagent

product line

ReagentPlus®

product line

-

product line

-


Still not finding the right product?


Skladovací třída

11 - Combustible Solids

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

PPE (osobní ochranné prostředky)

dust mask type N95 (US), Eyeshields, Gloves



Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumenty section.

Potřebujete-li pomoc, obraťte se na Zákaznická podpora

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů



Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. To dissolve DTNB which alcohol should use?

    1 answer
    1. This product is tested for solubility in Ethanol at 10 mg/mL. Solutions may also be prepared at 5mM in 100 mM sodium or potassium phosphate buffer, pH 7.2, containing 0.1 mM EDTA. Please see the link below to review the entire product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/414/707/d8130pis.pdf

      Helpful?

  4. what is the storage temperature for this chemical

    1 answer
    1. This product can be stored at room temperature.

      Helpful?

Reviews

No rating value

Active Filters