This formulation contains 100 mg/L of Magnesium Chloride Heptahydrate and 133 mg/L of Calcium Chloride Dihydrate. Please see the link below to review the full formulation of this and other Dulbecco's buffer products:
https://www.sigmaaldrich.com/product/sigma/d8662#product-documentation
D8662
Dulbecco’s Phosphate Buffered Saline
Liquid, with MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture
Akční nabídka
2 of 3 reviewers received a sample product or took part in a promotion
Synonyma:
DPBS
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| Vám/SKU | Dostupnost | Cena |
|---|---|---|
100 mL | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 429,00 Kč |
500 mL | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 879,00 Kč |
1 L | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 1 920,00 Kč |
O této položce
429,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Dovolte nám, abychom vám pomohliNázev produktu
Dulbecco′s Phosphate Buffered Saline, With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: no
glucose: no (Dextro)
shipped in
ambient
storage temp.
2-8°C
General description
Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture.
Application
Dulbecco′s Phosphate Buffered Saline has been used:
- as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
- to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
- to wash cells for protein isolation
D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
- as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
- to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
- to wash cells for protein isolation
Biochem/physiol Actions
Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions:
- provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
- serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance
- when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
- provides a buffering system to maintain the medium within the physiological pH range
1 of 1
Tato položka | |||
|---|---|---|---|
| description With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | description With calcium chloride and magnesium chloride, 10×, liquid, sterile-filtered, suitable for cell culture | description dry powder, DPBS Modified, without calcium, without magnesium, suitable for cell culture | description liquid, sterile-filtered, DPBS Modified 10X, without calcium, without magnesium, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | stem cell: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 400 | Quality Level 400 | Quality Level 500 | Quality Level 400 |
| form liquid | form liquid | form dry powder | form liquid |
| storage temp. 2-8°C | storage temp. room temp | storage temp. - | storage temp. - |
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility sterile-filtered |
| impurities endotoxin, tested | impurities - | impurities - | impurities - |
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Skladovací třída
12 - Non Combustible Liquids
Co se děje?
nwg
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
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What is the concentration of MgCl2 and CaCl2?
1 answer-
Helpful?
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Can this solution be autoclaved?
1 answer-
This solution is already sterile-filtered and can be autoclaved at 121°C, 15 psi for 20 minutes.
Helpful?
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Could you give me the precise composition of this DPBS buffer solution (mixture of different salts)
1 answer-
The composition of this product, Dulbecco's Phosphate Buffered Saline, is available in the following Product Information Sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/137/207/d8662for.pdf
Helpful?
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¿Disponen de algún estudio de estabilidad de este producto a temperatura Ambiente? (15 - 30 ºC)
1 answer-
Any stability study information is considered proprietary and may not be shared. However, this sterile DPBS solution is quite stable. The product is assigned an expiration date of approximately 2 years from the date of quality release. The item ships at ambient temperature and is stable for up to two weeks to allow for transit time. For long term storage, refrigeration at 2-8°C is recommended.
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