The stability of this compound in solution has not been evaluated. However, external sources indicate that stock solutions prepared in DMSO are stable for up to 6 months at -20°C when protected from light. Similar compound DCFHDA, product 35845, is stable in organic solvents for up to 6 months at -20°C, when protected from light.
F7378
Fluorescein diacetate
Powder
Synonyma:
3,6-Diacetoxyfluoran, Di-O-acetylfluorescein
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
Změnit zobrazení
| Vám/SKU | Dostupnost | Cena |
|---|---|---|
5 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 2 100,00 Kč |
10 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 4 070,00 Kč |
25 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 8 250,00 Kč |
100 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 25 700,00 Kč |
O této položce
Empirický vzorec (Hillův zápis):
C24H16O7
Číslo CAS:
Molekulová hmotnost:
416.38
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
209-877-6
MDL number:
Beilstein/REAXYS Number:
62575
2 100,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.
Dovolte nám, abychom vám pomohliNázev produktu
Fluorescein diacetate, used as cell viability stain
Quality Segment
form
powder
technique(s)
titration: suitable
color
white to faint yellow
mp
200-203 °C (lit.)
solubility
acetone: 25 mg/mL
ε (extinction coefficient)
≥86000 at 488-494 nm in 0.1 M NaOH
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1
InChI
1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
InChI key
CHADEQDQBURGHL-UHFFFAOYSA-N
General description
Lipase substrate
Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.
Application
Fluorescein diacetate has been used for the differentiation of live cells from dead cells.
1 of 1
Tato položka | |||
|---|---|---|---|
| description used as cell viability stain | description United States Pharmacopeia (USP) Reference Standard | description British Pharmacopoeia (BP) Reference Standard | description used as fluorescent tracer |
| technique(s) titration: suitable | technique(s) - | technique(s) - | technique(s) titration: suitable |
| color white to faint yellow | color - | color - | color - |
| application(s) diagnostic assay manufacturing | application(s) pharmaceutical (small molecule) | application(s) pharmaceutical | application(s) diagnostic assay manufacturing |
| form powder | form - | form solid | form powder |
| solubility acetone: 25 mg/mL | solubility - | solubility - | solubility H2O: 1 mg/mL |
| storage temp. room temp | storage temp. −20°C | storage temp. 2-8°C | storage temp. room temp |
Still not finding the right product?
Explore all of our products under Fluorescein diacetate
Skladovací třída
11 - Combustible Solids
Co se děje?
WGK 3
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
PPE (osobní ochranné prostředky)
Eyeshields, Gloves, type N95 (US)
Vyberte jednu z posledních verzí:
Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
-
How long can you store the FdA F7378 dissolved in acetone (5mg/ml) at -20°C?
1 answer-
Helpful?
-
-
Storage of the product
1 answer-
This product is stored at room temperature.
Helpful?
-
-
What solubility information do you have on Product F7378, Fluorescein diacetate?
1 answer-
We have found that it is slightly soluble in water at 0.1%, and in ethanol at 0.3%. It is also soluble in dimethylformamide (DMF).
Helpful?
-
-
What are some common applications for Product F7378, Fluorescein diacetate?
1 answer-
This compound is used extensively for the staining of living cells. It has been used as a viability stain for cultured neurons and plant ways, and in measuring of esterase and cholinesterase activity. See the following references for usage: Fluorometric determination of lipase: Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes", Anal. Chem. 36, 409, 1964. ; Anal. Biochem., Vol. 14, 28 (1966);Fluorescent stain for viable rosette-forming cells: J. Immun. Meth., Vol. 5,305 (1974);Fluorogenic substrate detection of viable pathogenic protozoa: Science, Vol. 227, 435 (1985);Reference Standard in antibody studies: Anal. Biochem., Vol. 9, 474 (1964);Cell viability staining: Staining Procedures, G. Clark, 4th ed., p. 93.Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes, Anal. Chem. 36, 409, 1964."" "
Helpful?
-
-
Is Product F7378, Fluorescein diacetate, a fluorescent compound?
1 answer-
No. Only upon hydrolysis of the esters, catalyzed by cellular esterase, the lactone ring opens yielding fluorescein. Only the fluorescein form is fluorescent.
Helpful?
-
-
How stable are solutions of Product F7378, Fluorescein diacetate, once prepared?
1 answer-
Based on our experience, stock solutions of fluorescein diacetate at 5 mg/mL in acetone or DMSO can be stored at -20 °C. According to one on-line source, solutions at 5 mg/mL in acetone or ethanol can be stored refrigerated.
Helpful?
-
-
After Product F7378, Fluorescein diacetate, has been converted to fluorescein, what are the maximum excitation and emission wavelengths?
1 answer-
The maximum excitation wavelength is 490 nm and the maximum emission wavelength is 526 nm.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-