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P5368

Phosphate buffered saline

powder, pH 7.4, BioPerformance Certified, for molecular biology

Synonyma:

PBS

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Vám/SKUDostupnostCena
10 pkg
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4 210,00 Kč

O této položce

UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25
MDL number:

4 210,00 Kč


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Název produktu

Phosphate buffered saline, BioPerformance Certified, pH 7.4

Quality Segment

grade

BioPerformance Certified, Molecular Biology

form

powder

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

technique(s)

immunofluorescence: suitable

impurities

BioBurden, tested, endotoxin, tested

color

white

pH

7.4

solubility

water: 9.07-10.0 g/L, clear, colorless

cation traces

Fe: ≤10 ppm, heavy metals (as Pb): ≤5 ppm

suitability

suitable for SDS-PAGE, suitable for molecular biology

application(s)

diagnostic assay manufacturing
histology
sample preparation

foreign activity

DNase, RNase, Protease, none detected

SMILES string

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

InChI key

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

Application


  • Rapid and zero-cost DNA extraction from soft-bodied insects for routine PCR-based applications.: This study used phosphate buffered saline (PBS) to develop a cost-effective and efficient method for DNA extraction from insects, facilitating routine PCR-based applications in molecular biology (Jangra & Ghosh, 2022).

  • Injectable amnion hydrogel-mediated delivery of adipose-derived stem cells for osteoarthritis treatment.: PBS was utilized to prepare and deliver adipose-derived stem cells in an injectable hydrogel, showing potential for innovative osteoarthritis treatments (Bhattacharjee et al., 2022).

  • Rapid and sensitive detection of SARS-CoV-2 infection using quantitative peptide enrichment LC-MS analysis.: This research employed PBS for sample preparation in a novel LC-MS method for detecting SARS-CoV-2, providing a rapid and sensitive diagnostic tool (Hober et al., 2021).

Packaging

Foil Pouch

Preparation Note

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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Tato položka
P381308057P3744
description

BioPerformance Certified, pH 7.4

description

powder, pH 7.4, for preparing 1 L solutions

description

BioUltra, pH 7.4 ( in solution), contains TWEEN 20, tablet

description

pH 7.6 (25 °C)

technique(s)

immunofluorescence: suitable

technique(s)

-

technique(s)

-

technique(s)

-

grade

Molecular Biology, BioPerformance Certified

grade

-

grade

-

grade

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

application(s)

diagnostic assay manufacturing
histology
sample preparation

application(s)

advanced drug delivery
diagnostic assay manufacturing
life science and biopharma
sample preparation

application(s)

-

application(s)

diagnostic assay manufacturing

form

powder

form

powder

form

tablet

form

powder

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

storage condition

dry at room temperature

storage condition

-

storage condition

-


Still not finding the right product?

Explore all of our products under Phosphate buffered saline


Skladovací třída

11 - Combustible Solids

Co se děje?

WGK 1

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable



Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–7 of 7 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the shelf-life of product P5368, phosphate buffered saline (PBS)?

    1 answer
    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. If reconstituted with sterile water, will Product P5368, Phosphate buffered saline (PBS), be sterile?

    1 answer
    1. No.  To make this PBS sterile, the solution should be either sterile-filtered or autoclaved.

      Helpful?

  6. What volume of Product P5368, Phosphate buffered saline (PBS), does each pack make?

    1 answer
    1. When the contents of a pouch is dissolved in 1 Liter of water, it makes 1X PBS, pH 7.4 at 25°C.

      Helpful?

  7. Does Product P5368, Phosphate buffered saline (PBS), contain calcium and magnesium?

    1 answer
    1. No.  The complete formulation is shown in the product listing.  When reconstituted, the resulting solution is 10 mM phosphate buffer, with 138 mM sodium chloride and 2.7 mM potassium chloride.

      Helpful?

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