The percentage of protein and units per mg of protein are reported in the batch-specific Certificate of Analysis. Multiplying these two values yields units per mg of solid. This value should be used to calculate activity when the powder is weighed out. For example, if the protein percentage is 10.4% and the units per mg is 1358 (lot 0000509386), the calculation would be as follows:
10.4% × 1358 = 141.232 units/mg solid
To prepare a dissolution medium containing 750,000 units/L, the calculation would be:
750,000 / 141.232 ≈ 5310.41 mg
Thus, approximately 5.3 grams of powder per liter is required.
P7125
Pepsin from porcine gastric mucosa
powder, ≥400 units/mg protein
Akční nabídka
Synonyma:
Pepsin A, Pepsin from hog stomach
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| Vám/SKU | Dostupnost | Cena |
|---|---|---|
100 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 2 840,00 Kč |
500 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 10 900,00 Kč |
O této položce
Číslo CAS:
UNSPSC Code:
12352204
eCl@ss:
42010127
EC Number:
232-629-3
NACRES:
NA.54
MDL number:
Specific activity:
≥400 units/mg protein
Biological source:
Porcine gastric mucosa
2 840,00 Kč
Ušetřete až 15 %
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Dovolte nám, abychom vám pomohlibiological source
Porcine gastric mucosa
Quality Segment
form
powder
specific activity
≥400 units/mg protein
mol wt
35 kDa
solubility
10 mM HCl: soluble 1.0 mg/mL, clear to faintly turbid, colorless
UniProt accession no.
storage temp.
2-8°C
Gene Information
pig ... LOC396892(396892)
Application
Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. Product P7125 is provided in powder form. Product P7125 has been used to denature DNA from kidney cells and to digest pathology samples from anal canal carcinomas (ACC) biopsies prior to EGFR staining.
The enzyme from Sigma has been used to obtain total vitamin B12 content in food products prior using immunoaffinity columns It has also been used to digest minced soft tissue of snails prior to the isolation of the third stage larvae (L3).
Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.
Biochem/physiol Actions
It does not cleave at valine, alanine, or glycine linkages. Z-L-tyrosyl-L-phenylalanine, Z-L-glutamyl-L-tyrosine, or Z-L-methionyl-L-tyrosine may be used as substrates for pepsin digestion. Pepsin is inhibited by several phenylalanine-containing peptides.
Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.
Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.
Analysis Note
Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.
Protein determined by E1%/280
Other Notes
One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)
View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.
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Tato položka | |||
|---|---|---|---|
| description powder, ≥400 units/mg protein | description tested according to Ph. Eur. | description lyophilized powder, ≥2,500 units/mg protein (E1%/280) | description lyophilized powder, ≥30 units/mg dry solid |
| biological source Porcine gastric mucosa | biological source - | biological source - | biological source Porcine gastric mucosa |
| specific activity ≥400 units/mg protein | specific activity - | specific activity ≥2,500 units/mg protein (E1%/280) | specific activity ≥30 units/mg dry solid |
| Gene Information pig ... LOC396892(396892) | Gene Information pig ... LOC396892(396892) | Gene Information pig ... LOC396892(396892) | Gene Information pig ... LOC396892(396892) |
| form powder | form powder | form lyophilized powder | form lyophilized powder |
| mol wt 35 kDa | mol wt 35 kDa | mol wt 35 kDa | mol wt 35 kDa |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Skladovací třída
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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9001-75-6
CAS No.
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How can I calculate the amount of pepsin P7125 needed to prepare a dissolution medium containing 750,000 units/L? Do I need to consider the reported protein percentage and the units/mg of protein to obtain the correct amount?
1 answer-
Helpful?
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How can I find out the protein content of each lot? I want to make a 1 mg pepsin protein (not powder) / ml solution. The CoA only shows protein content is greater than1; it does not state the actual protein content for each lot.
1 answer-
The % Protein and specific activity is published in the lot specific Certificate of Analysis (COA):
https://www.sigmaaldrich.com/product/sigma/p7125#product-documentationThe Specification Sheet is not lot specific, and only provides the testing criteria for each batch.
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Can you please tell me the Grade of this product?
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This falls under the research grade.
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Hi, What is the shelf life for this product? Thanks
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This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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Which Pepsin do you recommend for histology/antigen retrevial? According to protocol 0.2mg/ml in 0.2M HCL
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This product has not been specifically qualified for antigen retrieval, however, it may be able to be qualified for use by the end user. A typical protocol using pepsin for antigen retrieval suggests the following stock and working solutions:
Pepsin Stock Solution (1% in 10mM HCl):
Pepsin ------------------------------------- 100 mg
10mM HCl (pH 2.0) ---------------------- 10 mlMix to dissolve. Store at -20 ºC
Pepsin Working Solution (0.5% in 5mM HCl):
Pepsin Stock Solution (0.5%) ------------ 1 ml
Distilled water ---------------------------- 1 mlMix well.
For antigen retrieval - cover sections with pepsin working solution and incubate for 10-20 minutes at 37 °C in humidified chamber (optimal incubation time may vary depending on tissue type and degree of fixation, and should be determined by user).
Product R2283 is a pepsin product qualified for antigen retrieval https://www.sigmaaldrich.com/product/sigma/r2283.
For more information, please see the following link: https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/immunohistochemistry/immunohistochemistry-protocol#antigen_retrieval.
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