RDD002

HEPES

≥99.5%, crystalline powder, anhydrous, Redi-Dri^™

• Synonyma: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirický vzorec (Hillův zápis): C₈H₁₈N₂O₄S
• Číslo CAS: 7365-45-9
• Molekulová hmotnost: 238.30
• NACRES: NA.25
• PubChem Substance ID: 329824215
• UNSPSC Code: 12161700
• EC Number: 230-907-9
• MDL number: MFCD00006158
• Beilstein/REAXYS Number: 883043
• eCl@ss: 32129211

Vlastnosti

• Název produktu: HEPES, anhydrous, free-flowing, Redi-Dri^™, ≥99.5%
• grade: anhydrous
• Quality Segment: 100
• product line: Redi-Dri^™
• assay: ≥99.5%
• form: crystalline powder
• quality: free-flowing
• pH: 5.0-6.5 (25 °C, 238 g/L)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubility: water: 500 mg/mL, clear, colorless
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Popis

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:

• In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
• As a component in intracellular solution for the preparation of brain-slice recording
• As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
• To maintain the pH at 7 in thermal denaturation assay of purified protein
• In isocratic elution of protein using gel filtration
• Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Bezpečnostní informace

• Skladovací třída: 13 - Non Combustible Solids
• Co se děje?: WGK 1
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable