Hlavní dokumenty

Zobrazit celou dokumentaci

Přeskočit na

S2890

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii

Name: Streptavidin−Alkaline Phosphatase from <I>Streptomyces avidinii</I>
Brand: SIGMA

lyophilized powder, ≥700 DEA units/mg protein

Synonyma:

Streptavidin-AP, Streptavidin−AP from Streptomyces avidinii

Přihlásit pro zobrazení organizačních a smluvních cen.

Vybrat velikost

Změnit zobrazení

Vám/SKU	Dostupnost	Cena

O této položce

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00132384

Specific activity:

≥700 DEA units/mg protein

Informace o stanovených cenách a dostupnosti nejsou v současnosti dostupné.

Technický servis

Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.

Dovolte nám, abychom vám pomohli

Vlastnosti

conjugate

alkaline phosphatase conjugate

Quality Segment

200

form

lyophilized powder

specific activity

≥700 DEA units/mg protein

composition

Protein, ≥40% biuret

storage temp.

−20°C

Popis

General description

Streptavidin, a 58 kDa protein, is a homotetramer purified from Streptomyces avidinii. It has a high affinity for biotin. Streptavidin has been conjugated with either peroxidase or alkaline phosphatase (ALP). These enzymes are used as protein conjugates because of their comparative stability and a wide range of suitable substrates for different applications. ALP conjugates to streptavidin in immunoassays.

Application

The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/ml stock in buffer.

Streptavidin-alkaline phosphatase from Streptomyces avidinii has been used in enzyme-linked immune absorbent spot (ELISpot) to detect interferon (IFN-γ) producing cells.

Physical form

Lyophilized powder containing trehalose with EPPS, phosphate and traces of magnesium chloride and zinc chloride.

Other Notes

One alkaline phosphatase unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Porovnat podobné položky

Zobrazit úplné porovnání

Zobrazit rozdíly

1 of 1

Tato položka	S5512	189730	S0677
Sigma-Aldrich S2890 Streptavidin−Alkaline Phosphatase from Streptomyces avidinii	Sigma-Aldrich S5512 Streptavidin−Peroxidase from Streptomyces avidinii	Sigma-Aldrich 189730 Streptavidin	Sigma-Aldrich S0677 Streptavidin from Streptomyces avidinii
description lyophilized powder, ≥700 DEA units/mg protein	description lyophilized powder	description -	description recombinant, expressed in E. coli, lyophilized powder
specific activity ≥700 DEA units/mg protein	specific activity -	specific activity ≥10 units/mg protein	specific activity -
form lyophilized powder	form lyophilized powder	form lyophilized solid	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 200
composition Protein, ≥40% biuret	composition Protein, ≥70% E1%/280	composition -	composition -
conjugate alkaline phosphatase conjugate	conjugate peroxidase conjugate	conjugate -	conjugate -

Skladovací třída

11 - Combustible Solids

Co se děje?

WGK 3

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

PPE (osobní ochranné prostředky)

Eyeshields, Gloves, type N95 (US)

Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumenty section.

Potřebujete-li pomoc, obraťte se na Zákaznická podpora

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.