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CS0010

β-Secretase (BACE1) Activity Detection Kit (Fluorescent)

1 kit sufficient for 250 reactions

Synonym(e):

BACE1 Activity Detection Kit (Fluorescent)

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Größe/SKUVerfügbarkeitPreis
1 kit
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1.360,00 €

Über diesen Artikel

NACRES:
NA.84
UNSPSC Code:
12161503
eCl@ss:
32160701

1.360,00 €

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Quality Segment

usage

 kit sufficient for 250 reactions

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... BACE1(23621)

Application

The kit provides all the reagents required for an efficient detection of BACE1 activity. It contains an enzyme to be used for screening for potential BACE1 inhibitors. The assay is based on the fluorescence resonance energy transfer (FRET) method in which the fluorescence signal enhancement is observed after substrate cleavage by BACE1.

Biochem/physiol Actions

BACE1 is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer′s. BACE1 is a target for inhibitor drug discovery.

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Dieser Artikel
S4195RAB0623S4562
β-Secretase (BACE1) Activity Detection Kit (Fluorescent) 1 kit sufficient for 250 reactions

Sigma-Aldrich

CS0010

β-Secretase (BACE1) Activity Detection Kit (Fluorescent)

β-Secretase human recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), ≥10,000 units/mg protein

Sigma-Aldrich

S4195

β-Secretase human

Human BACE1 / Beta-secretase 1 ELISA Kit

Sigma-Aldrich

RAB0623

Human BACE1 / Beta-secretase 1 ELISA Kit

β-Secretase inhibitor ≥97% (HPLC), powder

Sigma-Aldrich

S4562

β-Secretase inhibitor

description

1 kit sufficient for 250 reactions

description

recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), ≥10,000 units/mg protein

description

-

description

≥97% (HPLC), powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

Gene Information

human ... BACE1(23621)

Gene Information

human ... BACE1(23621)

Gene Information

human ... BACE1(23621)

Gene Information

-

usage

 kit sufficient for 250 reactions

usage

-

usage

-

usage

-

Quality Level

300

Quality Level

200

Quality Level

-

Quality Level

200

Lagerklasse

10 - Combustible liquids

WGK

WGK 3

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

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Fragen

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  1. What is the PDB ID of enzyme used?

    1 Antwort

    1. This kit includes human BACE1/?-secretase enzyme for activity detection and inhibitor screening. The exact PDB ID for the enzyme is not specified, however the protein is identical to product S4195, UniProt P56817. Please see the links below to review information that may be helpful.

      UniProt:
      https://www.uniprot.org/uniprotkb/P56817/entry

      RCSB PDB page for UniProt P56817
      https://www.rcsb.org/groups/sequence/polymer_entity/P56817

      Also note that commonly cited human BACE1 structures include PDB IDs 2ZHT and 1W50, References:
      PDB ID 2ZHT: Human BACE1 crystal structure — https://www.rcsb.org/structure/2ZHT
      PDB ID 1W50: Human BACE apo structure — https://www.rcsb.org/structure/1W50

      Hilfreich?

  2. what is the concentration of BACE1 enzyme in micromolar?

    1 Antwort

    1. The B9059 BACE-1 product included in the kit, serves as a positive control. The Technical Bulletin notes that the concentration of this item is approximate 3 units/microliter. This material has not been tested for purity. Therefore, a molar concentration cannot be provided.

      Hilfreich?

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