D4635

2′-Desoxycytidin-5′-triphosphat Dinatriumsalz

≥95%

• Synonym(e): dCTP-Na₂
• Empirische Formel (Hill-System): C₉H₁₄N₃Na₂O₁₃P₃
• CAS-Nummer: 102783-51-7
• Molekulargewicht: 511.12
• NACRES: NA.51
• PubChem Substance ID: 24893889
• UNSPSC Code: 41106305
• MDL number: MFCD00084683
• Assay: ≥95%
• Form: powder
• Storage temp.: −20°C

Eigenschaften

• Quality Segment: 200
• assay: ≥95%
• form: powder
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2
• InChI: 1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1
• InChI key: ABWVCNMFYVEBIB-CDNBRZBRSA-L

Beschreibung

General description

2′-Deoxycytidine 5′-triphosphate (dCTP) is synthesized either by de novo pathway or from the multiple phosphorylation steps of cytidine. It is deaminated to deoxyuridine monophosphate (dUMP) which is convertible to deoxythymidine triphosphate (dTTP).

Application

2′-Desoxycytidin-5′-triphosphat-dinatriumsalz ist eines der Nukleotidtriphosphate, das für durch DNA-Polymerase ausgelöste Reaktionen wie z. B. Polymerase-Kettenreaktion, DNA-Sequenzierung und andere molekularbiologische Methoden verwendet wird.

2′-Deoxycytidine 5′-triphosphate disodium salt has been used:

• in polymerase chain reaction mix for the amplification of Alternaria (AM) toxin DNA[1]
• to spike oligonucleotide sample for capillary electrophoresis (CE) analysis[2]
• as a component in adenosine 5′-monophosphate (AMP) luciferase assay[3]

Biochem/physiol Actions

2′-Deoxycytidine 5′-triphosphate (dCTP) is used to synthesize DNA by DNA polymerases or reverse transcriptases. dCTP is an allosteric regulator of deoxycytidylate (dCMP) deaminase.[4] It is used in studies of nucleotide pools, nucleotide selectivity and enzyme specificity studies, such as the preferred incorporation of dCTP by Rev1, a γ-family DNA polymerase.[5]

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable