UPBSTRT

Ultra pure BST-RT Kit

Combined Ultra pure BST-RT polymerase, Ultra pure BST-RT Reaction Buffer and Magnesium sulfate

• Synonym(e): Polymerase, Reverse Transcriptase
• UNSPSC Code: 12352204
• eCl@ss: 32150313
• Technique(s): RT-qPCR: suitable (Isothermal amplification), cDNA synthesis: suitable
• Storage temp.: −20°C (−15°C to −25°C)
• Shipped in: dry ice

Eigenschaften

• grade: Molecular Biology
• Quality Segment: 400
• form: liquid
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): RT-qPCR: suitable (Isothermal amplification), cDNA synthesis: suitable
• impurities: (DNase free, RNasefree, DNA free, NICKase free)
• color: colorless
• suitability: suitable for PCR (Isothermal amplification)
• shipped in: dry ice
• storage temp.: −20°C (−15°C to −25°C)

Beschreibung

General description

BST-RT Polymerase is an engineered Bst enzyme with robust reverse transcriptase (RT) activity, making it ideal for use in loop-mediated isothermal amplification (LAMP) or RT-LAMP assays. This feature eliminates the necessity of a secondary RT enzyme when targeting amplification from RNA samples, simplifying reaction setup. BST-RT works optimally at 65°C and can be used for real time reaction monitoring with a fluorescent dye or in end point detection assays. BST-RT polymerase is tolerant to potential reaction inhibitors such as ethanol, extraction buffers, and saliva. This enzyme is also compatible with workflows that incorporate dUTP for elimination of reaction carryover contaminants.

Application

Loop-mediated isothermal amplification (LAMP) has emerged as a key alternative to polymerase chain reaction (PCR)-based techniques for amplifying nucleic acids. While conventional PCR employs a thermal cycler for repetitive cycling temperatures when amplifying, isothermal amplification techniques occur at a single and fixed temperature, allowing the use of simple,portable, and more robust instruments for fast and exponential amplification.

Features and Benefits

• Compatible with samples containing either DNA orRNA
• Ability to perform RT-LAMP without additional RT enzymes
• Lacks 5′ to 3′ exonuclease domain
• DNase free, RNase free, DNA free, and Nickase free
• Active from 25-65 °C (optimal at 65 °C)
• Unit Definition: One unit is the amount of enzyme required to incorporate 10 nmol of dNTP onto ssDNA template for 30 minutes at 65°C
• Also available in stand-alone, high concentrations and glycerol-free formulations