B5283

Anti-Rabbit IgG (γ-chain specific)−Biotin antibody, Mouse monoclonal

clone RG-96, purified from hybridoma cell culture

• Sinónimos: Monoclonal Anti-Rabbit IgG (γ-chain specific)
• NACRES: NA.46

Propiedades

• Nivel de calidad: 200
• origen biológico: mouse
• forma del anticuerpo: purified immunoglobulin
• antibody product type: secondary antibodies
• clon: RG-96, monoclonal
• formulario: buffered aqueous solution
• species reactivity: rabbit
• should not react with: chicken, guinea pig, goat, horse, sheep, rat, bovine, human, pig, feline, canine
• aplicaciones: direct ELISA: 1:150,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000; western blot (chemiluminescent): 1:200,000-1:400,000
• isotipo: IgG1
• conjugado: biotin conjugate
• enviado en: dry ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.

IgG (immunoglobulin G) antibody is secreted by B cells and is found in blood and extracellular fluids and is enriched in mother′s milk. The variable region of IgG antibody is specific to antigens and is highly conserved.

Aplicación

Monoclonal Anti-Rabbit IgG (γ-chain specific)-Biotin is suitable for ELISA at a working dilution of 1:150,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. The antibody was used in β-catenin ELISA at a dilution of 1:2000.
Anti-Rabbit IgG (γ-chain specific)-Biotin antibody, Mouse monoclonal has been used in β-Catenin enzyme immunoassay. It has also been used in immunohistochemistry for examining histology of mammary glands.

Acciones bioquímicas o fisiológicas

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Rabbit IgG (γ-chain specific) antibody allows for the binding of various labels such as avidin or streptavidin. IgG antibody has its function similar to IgM antibody in complement system activation.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• storage_class_code: 12 - Non Combustible Liquids
• WGK Alemania: nwg
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable