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HPLC Analysis of Nitroaromatic Compounds Using Ascentis® Express Biphenyl

HPLC Analysis of Nitroaromatic Compounds Using Ascentis® Express Biphenyl application for HPLC

Materiales

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Descripción
Precios

45969

2,4-Dinitrotoluene

analytical standard

47243

1,3,5-Trinitrobenzene solution

certified reference material, 1000 μg/mL in acetonitrile

64065-U

Ascentis® Express Biphenyl Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

CONDITIONS

column

Ascentis Express Biphenyl, 10 cm x 2.1mm I.D., 2.7 μm particles (64065-U)

mobile phase

[A]:Water; [B]:Methanol; (50:50, A:B)

flow rate

0.5 mL/min

pressure

7150 psi (492 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

1 μL

sample

Toluene, Nitrobenzene;1,3,5-Trinitrobenzene; 2,4-Dinitrotoluene.  100 μg/mL in 50:50 A:B

Descripción

Nota de análisis

This application demonstrates the separation of selected nitroaromatic compound using Ascentis Express Biphenyl. The biphenyl stationary phase is well suited to these substituted aromatic compounds due to interactions between the aromatic stationary phase and the electropositive benzene ring of the analyte. Unique selectivity is observed running this reversed phase separation using methanol mobile phase conditions

Información legal

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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