03-0160-00

SMC^® Human IL-1β High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IL-1β in human serum and plasma

• Synonym(s): IL-1 beta Single Molecule Counting Kit, IL-1 beta immunoassay kit, IL-1 beta ultra-sensitive immunoassay, Interleukin-1 beta immunoassay kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• product line: SMC^®
• usage: kit sufficient for 96 wells
• species reactivity: human
• manufacturer/tradename: SMC^®
• technique(s): single molecule counting: suitable
• input: plasma (K2 EDTA); serum
• UniProt accession no.: P01584
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Interleukin-1 beta (IL-1β) is a pro-inflammatory cytokine produced mainly by activated macrophages and other immune cells. It plays a pivotal role in mediating inflammatory responses, promoting the activation of immune cells, and influencing the production of other cytokines. IL-1β is also involved in various physiological processes, including fever induction and the regulation of immune system responses, making it a key player in both health and disease.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Biochem/physiol Actions

Although IL-1β was detectable in non-human primate, rat, equine, feline and canine samples, the assay has not been verified for these species.

Features and Benefits

• High-Performance Sensitivity: The SMC^®technology excels in the quantification of IL-1β in human serum and plasma, enabling highly accurate measurements.
• Built for Confidence: With low variability and high reproducibility, you can trust your data across experiments. Whether you′re profiling inflammation or validating biomarkers, this assay delivers consistent, high-quality results.
• Deeper Insights into Inflammation: IL-1β is a key player in autoimmune diseases, neuroinflammation, and sepsis. This assay helps you explore its role with unmatched clarity.
• Early Biomarker Detection: The ultra-sensitive performance supports early-stage discovery and therapeutic development.

Packaging

96 well assay

Analysis Note

• Sensitivity: LLOQ 200 fg/mL* (SMCxPRO^® Instrument
• Standard Curve Range: 50-50,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Serum: 107% Plasma: 111%
• Recovery: Serum: 85% Plasma: 83%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Inflammation & Immunology Inflammatory Disease Immune Response Autoimmune Disease

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H319,H360FD,H412
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects