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Microcon-30kDa Centrifugal Filter Unit with Ultracel-30 membrane

This 0.5 mL centrifugal filter enables the concentration of biomolecules with a membrane NMWL of 30 kDa. Micron centrifugal filters are ideal for DNA & protein concentration & desalting.



Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade)

Quality Level




holdup volume> 10 μL




DNA purification: suitable
RNA purification: suitable
protein extraction: suitable
protein purification: suitable


4.50 cm (1.8 in.)


12.3 mm

filtration area

0.32 cm2

pore size

30 kDa NMWCO

test parameters

sample volume: 0.5 mL

shipped in


General description

Simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. With the low-binding Ultracel membrane, Microcon filters offer:
•Typical recoveries of greater than 95%, even for dilute solutions
•Reverse spin to maximize recovery, even in the smallest samples
•Convenient storage of filtrate or concentrated sample in standard microfuge tube
•Concentration factors up to 100X

•High recovery for small volumes with reverse spin (concentration factor less than 20X)
•Low-binding Ultracel membrane
•Fast processing

Sample applications:
•Recovery of genomic DNA for forensic applications
•Concentration and desalting of nucleic acids (high recovery alternative to ethanol precipitation)
•Removal of primers from amplified DNA
•Easy filtrate recovery, exploiting the flat membrane design


Replaces: 42410

Legal Information

MICROCON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13

Certificate of Analysis

Certificate of Origin

Flavio Oliveira et al.
Toxins, 12(3) (2020-04-05)
Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species...
Sergey Radko et al.
Biomedicines, 8(5) (2020-05-28)
Selected reaction monitoring (SRM) is a mass spectrometric technique characterized by the exceptionally high selectivity and sensitivity of protein detection. However, even with this technique, the quantitative detection of low- and ultralow-abundance proteins in blood plasma, which is of great...
Michael D Ward et al.
Clinical proteomics, 17, 11-11 (2020-03-21)
Detection of viral ribo-nucleic acid (RNA) via real-time polymerase chain reaction (RT-PCR) is the gold standard for the detection of Ebola virus (EBOV) during acute infection. However, the earliest window for viral RNA detection in blood samples is 48-72 h post-onset...
Sarah K Stumper et al.
eLife, 8 (2019-02-06)
RNA polymerases (RNAPs) contain a conserved 'secondary channel' which binds regulatory factors that modulate transcription initiation. In Escherichia coli, the secondary channel factors (SCFs) GreB and DksA both repress ribosomal RNA (rRNA) transcription, but SCF loading and repression mechanisms are...
Chung-Hsuan Kao et al.
Molecular and cellular biology, 40(12) (2020-03-25)
Maintenance of protein homeostasis in eukaryotes under normal growth and stress conditions requires the functions of Hsp70 chaperones and associated cochaperones. Here, we investigate an evolutionarily conserved serine phosphorylation that occurs at the site of communication between the nucleotide-binding and...


Development of a Simple and Rapid Digestion Protocol for Proteomics Sample Preparation

In this study, we developed a rapid trypsin digest kit that, at elevated temperatures, yielded reliable, reproducible results in less than 2 hours on a wide variety of substrates for mass spectrometry.

A Rapid, Streamlined Workflow for Glycan Sample Preparation and Analysis by LC-MS

The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.

Related Content

Microcon® Centrifugal Filters Applications Guidelines & Specifications

Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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