MRCF0R100ET
Microcon® DNA Fast Flow Centrifugal Filters
sample volume 0.5 mL, Ultracel® regenerated cellulose membrane (low binding), Ethylene Oxide (EtO) treated
Synonym(s):
Centrifuge concentrator
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material
Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade, dual cycle ethylene oxide (EtO) treated)
Quality Level
sterility
non-sterile
feature
holdup volume> 10 μL
packaging
pkg of 20 ea
manufacturer/tradename
Microcon®
parameter
0.5 mL sample volume
technique(s)
DNA purification: suitable
RNA purification: suitable
L
4.50 cm (1.8 in.)
diam.
12.3 mm
filtration area
0.32 cm2
working volume
0.5 mL
shipped in
ambient
Related Categories
1 of 4
This Item | MRCF0R100 | MRCF0R030 | MRCPRT010 |
|---|---|---|---|
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| material Ultracel® regenerated cellulose membrane (low binding), polycarbonate device top, silicone O-ring (medical-grade, dual cycle ethylene oxide (EtO) treated), polyacetal base (membrane support), polypropylene tube (for filtrate/concentrate) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade) |
| manufacturer/tradename Microcon® | manufacturer/tradename Microcon® | manufacturer/tradename Microcon® | manufacturer/tradename Microcon® |
| parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume |
| feature holdup volume> 10 μL | feature holdup volume> 10 μL | feature holdup volume> 10 μL | feature holdup volume> 10 μL |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable, RNA purification: suitable | technique(s) DNA purification: suitable, RNA purification: suitable, protein extraction: suitable, protein purification: suitable | technique(s) DNA purification: suitable, RNA purification: suitable, protein extraction: suitable, protein purification: suitable |
General description
•Typical recoveries of greater than 95%, even for dilute solutions
•Reverse spin to maximize recovery, even in the smallest samples
•Convenient storage of filtrate or concentrated sample in standard microfuge tube
•Concentration factors up to 100X
Microcon DNA Fast Flow Filter
Optimized for the concentration and recovery of genomic DNA with SDS buffer. The low nonspecific binding characteristics of the membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss.
Features:
•High recovery for small volumes with reverse spin (concentration factor less than 20X)
•Low-binding Ultracel membrane
•Fast processing
Sample applications:
•Recovery of genomic DNA for forensic applications
•Concentration and desalting of nucleic acids (high recovery alternative to ethanol precipitation)
•Removal of primers from amplified DNA
•Easy filtrate recovery, exploiting the flat membrane design
Features and Benefits
Physical form
Legal Information
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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Related Content
Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.
The new dual cycle EtO-treated Microcon® DNA Fast Flow PCR Grade devices enable centrifugal concentration at a similar rate as the Microcon® DNA Fast Flow devices, but the EtO treatment greatly reduces the risk that no uncontrolled contaminating nucleic acids enter the analysis process. Both Microcon® devices exhibit faster flow rate (volume filtered per minute) compared to competitor centrifugal products.
Maximum recovery of pure intact genomic DNA from crude biological samples is the critical first step in any forensic analysis workflow. Typically, the process involves cell disruption, chemical or ion exchange-based extraction of the nucleic acid fraction and concentration of the purified DNA sample. While ethanol precipitation is commonly used for DNA concentration in research settings, an effective alternative is ultrafiltration via small spin columns such as the Microcon® DNA Fast Flow device. However, one overriding concern in the field of genetic testing is consumable product contamination with exogenous DNA during their assembly and packaging. To minimize this potential, a dual cycle ethylene oxide (EtO) treatment is employed to disrupt or fragment amplifiable DNA, thereby reducing the risk of false positives or uninterpretable results. To meet this concern, EMD Millipore has released the Microcon® DNA Fast Flow PCR Grade filter, an EtO-treated form of the parent device that has been shown here to match the original Microcon® device in performance.
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