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L7275

Sigma-Aldrich

LB Broth (Lennox)

Tablet microbial growth medium

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Synonym(s):
Lennox broth , Luria Bertani Broth
NACRES:
NA.85

grade

for molecular biology

Quality Level

200

sterility

non-sterile

form

tablet

application(s)

food and beverages

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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This Item
L3022L7658L2897
LB Broth (Lennox) Tablet microbial growth medium

Sigma-Aldrich

L7275

LB Broth (Lennox)

LB Broth (Lennox) Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

Sigma-Aldrich

L3022

LB Broth (Lennox)

LB Broth (Lennox) EZMix™ powder microbial growth medium

Sigma-Aldrich

L7658

LB Broth (Lennox)

LB Broth with agar (Lennox) Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

Sigma-Aldrich

L2897

LB Broth with agar (Lennox)

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

form

tablet

form

powder

form

powder

form

-

application(s)

food and beverages

application(s)

food and beverages

application(s)

agriculture
food and beverages

application(s)

food and beverages

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

Dehydrated Luria-Bertani (LB) broth is called the Lennox LB, which has amino acids as carbon source.
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Application

LB Broth (Lennox) has been used for culturing Escherichia coli and bacterial artificial chromosomes (BACs).
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Features and Benefits

Lennox LB tablets provide:
  • Convenient small package to eliminate weighing
  • Fast preparation – no stirring required

Components

10 g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
2.2 g/L inert binding agents

Reconstitution

Add each 1.1g tablet in 48.3mL water (no stirring required). Autoclave for 20 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Legal Information

Zeocin is a registered trademark of Cayla Sarl

related product

Product No.
Description
Pricing

L7025

LB Broth with agar (Lennox), Tablet microbial growth medium

L7658

LB Broth (Lennox), EZMix™ powder microbial growth medium

L7533

LB Broth with agar (Lennox), EZMix™ powder microbial growth medium

L3022

LB Broth (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

L2897

LB Broth with agar (Lennox), Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

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EnPresso® B, Growth system for expressing protein in bacteria

CMC0001

SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production

CMC0007

SIG10 5a Chemically Competent Cells, for DNA plasmid production

CMC0014

BL21(DE3) Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Simultaneous quantification of active carbon-and nitrogen-fixing communities and estimation of fixation rates using fluorescence in situ hybridization and flow cytometry
McInnes AS, et al.
Applied and Environmental Microbiology, 80(21), 6750-6759 (2014)
Fluorescence in situ hybridization of ductal lavage samples identifies malignant phenotypes from cytologically normal cells in women with breast cancer
Adduci KM, et al.
Cancer Cytopathology, 111(3), 185-191 (2007)
Hybridization between Escherichia coli and Shigella.
Luria, S.E. and Burrous, J.W.
Journal of Bacteriology, 74, 281-281 (1957)
In Vitro packaging of λ dam vectors and their use in cloning DNA fragments.
Enquist, L. and Sternberg, N.
Methods in Enzymology, 68, 281-281 (1971)
Miller, J.H.
Experiments in Molecular Genetics null
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