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Merck

58298

SUPELCOSIL LC-18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
NB.21
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Product Name

SUPELCOSIL LC-18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

agency

suitable for USP L1

Quality Level

feature

endcapped

manufacturer/tradename

SUPELCOSIL

extent of labeling

11.0% carbon loading

parameter

≤70 °C temp. limit
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 3.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

120 Å

pH

2-7.5

application(s)

food and beverages

separation technique

reversed phase

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General description

A general purpose hydrophobic alkyl phase that is very retentive and gives good peak shape for a wide variety of compounds.

Application

SUPELCOSIL LC-18 HPLC Column may be used for the separation of:
  • Anthocyanins, flavonols, and carbohydrates in Tropea red onions (Allium cepa L.) during their determination by high performance liquid chromatography (HPLC) as well as HPLC coupled to mass spectrometry (MS) equipped with electrospray ionization (ESI) source.
  • Sinapyl derivatives in pineapple (Ananas comosus [L.] Merill) juice samples during their determination by semipreparative reversed phase HPLC.
  • Aflatoxin M1 in milk and cheese samples during its determination by HPLC with post-column derivatization and fluorescence detection.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Kelas Penyimpanan

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Vagish Dwibedi et al.
Biotechnology letters, 43(3), 627-644 (2020-11-08)
The present investigation primarily focusses on enhancement of resveratrol production by endophytic production from the endophytic fungus, Arcopilus aureus via one variable at a time approach (OVAT) followed by statistical approach using response surface methodology (RSM). The paper also highlights
Characterization of sinapyl derivatives in pineapple (Ananas comosus [L.] Merill) juice.
Wen L, et al.
Journal of Agricultural and Food Chemistry, 47(3), 850-853 (1999)
High-performance liquid chromatography with post-column derivatisation and fluorescence detection for sensitive determination of aflatoxin M1 in milk and cheese.
Manetta AC, et al.
Journal of Chromatography A, 1083(1-2), 219-222 (2005)
Flavonoid and carbohydrate contents in Tropea red onions: effects of homelike peeling and storage.
Gennaro L, et al.
Journal of Agricultural and Food Chemistry, 50(7), 1904-1910 (2002)

Protokol

HPLC Analysis of Fatty Acid Methyl Esters (FAMES) on SUPELCOSIL™ LC-18

Separation of δ-Tocopherol, analytical standard; Retinyl acetate; (+)-γ-Tocopherol; α-Tocopherol, ≥95.5%; Retinol; Vitamin A (acetate), meets USP testing specifications.

Kromatogram

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