The thickness of the PTFE membrane for the PICM01250 inserts is approximately 65 micrometers (um), and these inserts are compatible with inverted microscopes.
PICM01250
Millicell® 24 well standing inserts
pore size 0.4 μm, diam. 12 mm, transparent PTFE membrane, hydrophilic, sterile
Synonym(s):
cell culture inserts, permeable culture insert, tissue culture insert, tissue culture plate
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50 PKG
₪2,256.00
₪2,256.00
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About This Item
UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22
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material
polystyrene housing
transparent PTFE membrane
Quality Level
sterility
ethylene oxide treated
sterile
feature
hydrophilic
packaging
pack of 50 (individually blister packaged)
manufacturer/tradename
Millicell®
parameter
50 °C max. temp.
technique(s)
cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
H
10.5 mm
diam.
12 mm
filtration area
0.6 cm2
size
24 wells
surface area
0.6 cm2
working volume
0.4 mL
color
transparent membrane, when wetted
matrix
Biopore™
pore size
0.4 μm
binding type
low binding surface
detection method
fluorometric
shipped in
ambient
1 of 4
This Item | PICM03050 | PIHA01250 | PI8P01250 |
|---|---|---|---|
| binding type low binding surface | binding type low binding surface | binding type high binding surface | binding type Tissue Culture (TC)-treated surface |
| sterility ethylene oxide treated, sterile | sterility ethylene oxide treated, sterile | sterility ethylene oxide treated, sterile | sterility γ-irradiated, sterile |
| material polystyrene housing, transparent PTFE membrane | material polystyrene housing, transparent PTFE membrane | material polystyrene housing, white mixed cellulose esters (MCE) membrane | material polystyrene housing, translucent polycarbonate membrane |
| manufacturer/tradename Millicell® | manufacturer/tradename Millicell® | manufacturer/tradename Millicell® | manufacturer/tradename Millicell® |
| pore size 0.4 μm | pore size 0.4 μm | pore size 0.45 μm | pore size 8.0 μm |
| feature hydrophilic | feature hydrophilic | feature hydrophilic | feature hydrophilic, 0.1x106 pores/cm2 (pore density) |
General description
With Millicell® standing inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® standing inserts make it possible to study both sides of the cell monolayer.
Application
- Cell Attachment
- Cell Growth
- Cell Differentiation
- Immunocytochemistry
Features and Benefits
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies
- Low protein-binding hydrophilic PTFE membrane is ideal for live cell viewing and immunofluorescent application
Legal Information
BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany
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Description
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ming Zeng et al.
Gene, 753, 144810-144810 (2020-05-30)
Meiotic entry and progression require dynamic regulation of germline gene expression. m6A on mRNAs and recognition by YTHDC2 has been known as post-transcriptional regulatory complex, but the roles of this regulator remain unclear for meiotic initiation and progression in female
J M Ogilvie et al.
Journal of neuroscience methods, 87(1), 57-65 (1999-03-05)
Organ culture systems of the central nervous system have proven to be useful tools for the study of development, differentiation, and degeneration. Some studies have been limited by the inability to maintain the cultures over an extended period. Here we
P Hardman et al.
Development, growth & differentiation, 35(6), 683-690 (1993-01-01)
Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane
R Le Bouffant et al.
Human reproduction (Oxford, England), 25(10), 2579-2590 (2010-07-31)
The initiation of meiosis is crucial to fertility. While extensive studies in rodents have enhanced our understanding of this process, studies in human fetal ovary are lacking. We used RT-PCR and immunohistochemistry to investigate expression of meiotic factors in human
Andrea S Cupp et al.
Biology of reproduction, 68(6), 2033-2037 (2003-02-28)
The first morphological event after initiation of male sex determination is seminiferous cord formation in the embryonic testis. Cord formation requires migration of pre-peritubular myoid cells from the adjacent mesonephros. The embryonic Sertoli cells are the first testicular cells to
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Protocols
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.
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