Skip to Content
Merck

M7167

M2 medium

With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

Synonym(s):

Cell Culture M2, Cell Culture Medium, M2 Medium Solution

Slide 1 of 1
Sign In to View Organizational & Contract Pricing.

Select a Size

50 ML

₪290.00

100 ML

₪434.00

₪290.00

Please contact Customer Service for Availability
Request a Bulk Order

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00283761

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

M2 medium, With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

sterility

sterile-filtered

form

liquid

storage condition

protect from light

technique(s)

cell culture | embryo: suitable

impurities

endotoxin, tested

components

HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L

shipped in

ambient

storage temp.

2-8°C

Quality Level

300

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
M729214660C14610C
M2 medium With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

Sigma-Aldrich

M7167

M2 medium

M16 Medium With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

Sigma-Aldrich

M7292

M16 Medium

EX-CELL® Sp2/0 Serum-Free Medium for Sp2/0 Cells Animal-component free, Protein free, Chemically defined, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

SAFC

14660C

EX-CELL® Sp2/0 Serum-Free Medium for Sp2/0 Cells

SAFC

SAFC

14610C

EX-CELL® 610-HSF Serum-Free Medium for Hybridoma Cells

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | embryo: suitable

technique(s)

cell culture | embryo: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

components

HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L

components

NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)

components

L-glutamine: no
glucose: 4.0 g/L
HEPES: 7.5 mM
NaHCO3: 1.05 g/L

components

glucose: 3.45 g/L
NaHCO3: 1.60 g/L
L-glutamine: 0.333 g/L
HEPES: 7.5 mM

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Analysis Note

Presence of particulates in solution will not affect product′s performance.
This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

Application

Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.

Preparation Note

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000450067
0000450067
0000450063
0000412807
0000412810

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Maryse Lessard et al.
Scientific reports, 9(1), 13829-13829 (2019-09-27)
The paternal environment is thought to influence sperm quality and future progeny may also be impacted. We hypothesized that prenatal exposure to environmentally-relevant contaminants impairs male reproduction, altering embryo gene expression over multiple generations. Folic acid (FA) can improve sperm
Hongzheng Sun et al.
Biology of reproduction, 100(3), 601-617 (2018-09-29)
Methionine adenosyltransferase II (MAT2A) is essential to the synthesis of S-adenosylmethionine, a major methyl donor, from L-methionine and ATP. Upon fertilization, zygotic genome activation (ZGA) marks the period that transforms the genome from transcriptional quiescence to robust transcriptional activity. During
Ludwig Stenz et al.
PloS one, 14(6), e0208371-e0208371 (2019-06-11)
Di(2-ethylhexyl)phthalate (DEHP) interferes with sex hormones signaling pathways (SHP). C57BL/6J mice prenatally exposed to 300 mg/kg/day DEHP develop a testicular dysgenesis syndrome (TDS) at adulthood, but similarly-exposed FVB/N mice are not affected. Here we aim to understand the reasons behind
Yi-Wen Xu et al.
Journal of cell science, 130(19), 3297-3307 (2017-08-19)
Precise regulation of DNA replication and genome integrity is crucial for gametogenesis and early embryogenesis. Cullin ring-finger ubiquitin ligase 4 (CRL4) has multiple functions in the maintenance of germ cell survival, oocyte meiotic maturation, and maternal-zygotic transition in mammals. DDB1-cullin-4-associated
Tao Fu et al.
FEBS letters, 593(15), 2040-2050 (2019-06-04)
Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family, which functions in embryo implantation and decidualization. The expression, function and regulation of Osm in mouse uteri during early pregnancy remain unknown. We show that Osm is mainly expressed
View All Related Papers

Articles

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Related Content

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service