This product is soluble in water (20 to 50 mg/ml) and in 0.1 N acetic acid (20 mg/ml). The filter used was CA (cellulose acetate, PES, Nylon, and PVDF). It is recommended to use low protein binding filter. Here are the protein binding characteristics for syringe filter materials:
A8052
Arg-Gly-Asp
≥97% (TLC)
Sinonimo/i:
L-arginyl-glycyl-L-aspartic acid
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1 MG
42,16 €
5 MG
161,00 €
10 MG
297,00 €
42,16 €
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Informazioni su questo articolo
Formula empirica (notazione di Hill):
C12H22N6O6
Numero CAS:
Peso molecolare:
346.34
Numero MDL:
Codice UNSPSC:
12352209
ID PubChem:
NACRES:
NA.32
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Servizio Tecnico
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Permettici di aiutartiOrigine biologica
synthetic
Saggio
≥97% (TLC)
Stato
powder
tecniche
cell culture | stem cell: suitable
N° accesso UniProt
Temperatura di conservazione
−20°C
Stringa SMILE
N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(O)=O
InChI
1S/C12H22N6O6/c13-6(2-1-3-16-12(14)15)10(22)17-5-8(19)18-7(11(23)24)4-9(20)21/h6-7H,1-5,13H2,(H,17,22)(H,18,19)(H,20,21)(H,23,24)(H4,14,15,16)/t6-,7-/m0/s1
IYMAXBFPHPZYIK-BQBZGAKWSA-N
Informazioni sul gene
human ... FN1(2335)
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Questo articolo | G1269 | A9041 | G4391 |
|---|---|---|---|
| assay ≥97% (TLC) | assay ≥97% (HPLC) | assay ≥95% (HPLC) | assay ≥97% (HPLC) |
| biological source synthetic | biological source human | biological source synthetic | biological source - |
| technique(s) cell culture | stem cell: suitable | technique(s) blocking: suitable, ligand binding assay: suitable | technique(s) cell culture | mammalian: suitable | technique(s) blocking: suitable, cell culture | mammalian: suitable |
| form powder | form powder | form powder | form powder |
| Gene Information human ... FN1(2335) | Gene Information human ... ITGA2B(3674), ITGAV(3685) | Gene Information human ... ITGA2B(3674), ITGB3(3690) | Gene Information human ... ITGB3(3690) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Amino Acid Sequence
Arg-Gly-Asp
Descrizione generale
Applicazioni
Arg-Gly-Asp has been used:
- to saturate the cell surface Arg-Gly-Asp (RGD) receptors and inhibit marrow stromal cell (MSC) attachment to peptide modified hydrogel[4]
- in adhesion assay[5]
- in inhibition assay to confirm the β1-integrin-dependency of cell adhesion[6]
- in textile fabrication and RGD textile functionalization[7]
- to engineer a population of human dermal fibroblasts with impaired migration[8]
- to modify glass substrate and evaluate the behavior of human bone marrow mesenchymal stem cells (hBM-MSCs)[9]
- in the cell culture of human mammary epithelial cells[10]
Azioni biochim/fisiol
Primary sequence involved with the binding of proteins to cell surfaces
Confezionamento
Bottomless glass bottle. Contents are inside inserted fused cone.
Nota sulla preparazione
Tripeptide from the cell-attachment domain of fibronectin
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Engineering the migration and attachment behaviour of primary dermal fibroblasts
Garcia-Gareta E, et al.
Biotechnology and Bioengineering (2019)
The effect of incorporating RGD adhesive peptide in polyethylene glycol diacrylate hydrogel on osteogenesis of bone marrow stromal cells
Yang F, et al.
Biomaterials, 26(30), 5991-5998 (2005)
Targeted and theranostic applications for nanotechnologies
Nanotechnologies in Preventive and Regenerative Medicine: An Emerging Big Picture, 399-399 (2017)
CCN2 promotes keratinocyte adhesion and migration via integrin alpha5beta1
Kiwanuka E, et al.
Experimental Cell Research, 319(19), 2938-2946 (2013)
Stijn F M van Dongen et al.
Advanced materials (Deerfield Beach, Fla.), 25(12), 1687-1691 (2013-01-29)
There's an APP for that: cell-repellent APP (azido-[polylysine-g-PEG]) is used to create substrates for spatially controlled dynamic cell adhesion. The simple addition of a functional peptide to the culture medium rapidly triggers cell adhesion. This highly accessible yet powerful technique
Contenuto correlato
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Can this peptide be filtered through a 0.2 um filter?
1 risposta-
Utile?
-
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Stability of RGD in DNAase-free water and stored at -80 up to what time it remain stable?
1 risposta-
The solution stability of this product has not been evaluated. Several commercial sources have reported that RGD peptides are stable up to 3 months at 4 deg. C. Other sources note that solutions are only stable for 24 hours. No information is available on storage at -80 C. The solution storage of this product would have to be determined by the end user.
Utile?
-
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