This product is a set of two tablets - one DAB tablet and one Urea Hydrogen Peroxide tablet. When prepared according to instructions, the solution should be used within one hour for best results.
D4293
3,3′-Diaminobenzidine
SIGMAFAST™, chromogenic, tablet
Sinonimo/i:
DAB tablets
Autenticati per visualizzare i prezzi organizzativi e contrattuali.
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
5 sets | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 75,60 € |
50 sets | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 380,00 € |
5 x 50 set | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 1020,00 € |
Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
75,60 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Servizio Tecnico
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Permettici di aiutartiNome del prodotto
SIGMAFAST™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 5 mL
Quality Segment
form
tablet
technique(s)
protein detection: suitable
storage temp.
−20°C
SMILES string
Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2
InChI key
HSTOKWSFWGCZMH-UHFFFAOYSA-N
Application
SIGMAFAST™ 3,3′-Diaminobenzidine tablets has been used as a chromogen for visualization in immunohistochemistry of gonad tissue, thymus, dental chronic periapical lesions and gall bladder sections.
Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets
Preparation Note
Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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Questo articolo | |||
|---|---|---|---|
| description tablet, To prepare 5 mL | description 97% (HPLC) | description tablet, To prepare 15 mL | description 97% |
| technique(s) protein detection: suitable | technique(s) - | technique(s) immunodetection: suitable | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form tablet | form powder | form tablet | form powder |
| storage temp. −20°C | storage temp. room temp | storage temp. −20°C | storage temp. - |
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Parola di avvertenza
Danger
Codici di pericolo
Avvertenze di sicurezza
Hazard Classifications
Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Classe di stoccaggio
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
WGK
WGK 3
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Hello, your product D4293, SIGMAFAST™ 3,3′-Diaminobenzidine tablets, can be used for how many months after dissolving in 5ml of distilled water?
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Hello, Can https://www.sigmaaldrich.com/US/en/product/sigma/d4293?icid=sharepdp-clipboard-copy-productdetailpage this product be used to develop western blots? Thank you, Regards Vanda
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D4293, SIGMAFAST™ 3,3′-Diaminobenzidine tablets, can be used for WB on PVDF membranes, please see attached reference paper stating below: "The PVDF was then collected and rinsed 3 times for 5–10 min each wash with TBS. The blot was then developed appropriately with 3',3-Diaminobenzidine tablets for developing peroxidase (D4293 Sigma-Aldrich) and BCIP/NBT for alkaline phosphatase (B5655 Sigma-Aldrich) and the blot was imaged."Scientific Neurogenic marker expression in differentiating human adipose derived adult mesenchymal stem cells Neus Gomila Pelegri1,2, Bruce K. Milthorpe1 , Catherine A. Gorrie2 , Jerran Santos1. Stem Cell Investig 2023;10:7
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