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Merck

E6758

Ethylenediaminetetraacetic acid

anhydrous, crystalline, BioReagent, suitable for cell culture

Sinonimo/i:

(Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid

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100 G

56,80 €

500 G

174,00 €

56,80 €


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Informazioni su questo articolo

Formula condensata:
(HO2CCH2)2NCH2CH2N(CH2CO2H)2
Numero CAS:
Peso molecolare:
292.24
NACRES:
NA.25
PubChem Substance ID:
eCl@ss:
39030907
UNSPSC Code:
12161700
EC Number:
200-449-4
MDL number:
Beilstein/REAXYS Number:
1716295

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Nome del prodotto

Ethylenediaminetetraacetic acid, anhydrous, crystalline, BioReagent, suitable for cell culture

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

grade

anhydrous

product line

BioReagent

assay

≥98.5%

form

crystalline

reaction suitability

reagent type: chelator

technique(s)

cell culture | mammalian: suitable

pH

2.5 (23 °C, 10 g/L)

mp

250 °C (dec.) (lit.)

solubility

3  M NaOH: 100 mg/mL

density

1.46 g/cm3 at 20 °C

cation traces

C: 40.7-41.5%
N: 9.3-9.8%

Quality Level

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EDSEDE9884
grade

anhydrous

grade

anhydrous

grade

purified grade

grade

ACS reagent

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

assay

≥98.5%

assay

≥99% (titration)

assay

≥98.5%

assay

99.4-100.6%

product line

BioReagent

product line

BioUltra

product line

-

product line

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

mp

250 °C (dec.) (lit.)

mp

250 °C (dec.) (lit.)

mp

250 °C (dec.) (lit.)

mp

250 °C (dec.) (lit.)

Application

Ethylenediaminetetraacetic acid has been used:
  • as a component of peptide dilution buffer for intracellular BH3 (Bcl-2 homology (BH) domain) profiling and validation[1]
  • as a medium component in the hepatic stellate cell (HSCs) culture[2]
  • as a component of homogenization buffer for protein extraction from eye tissues[3]
  • for stopping benzonase reaction in lysis buffer[4]

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, majorly used in preventing clotting of blood samples.[5] EDTA may trigger aggregation of platelets resulting in pseudothrombocytopenia.[6] EDTA along with tetracycline imparts serves as root conditioning agent and provides relief in gum infection.[5] EDTA-tetracycline therapy decreases coronary artery calcium levels in atherosclerosis.[7] Calcium Disodium EDTA administration is effective in slowing down the progression of chronic kidney disease.[8]

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Certificati d'analisi (COA)

Lot/Batch Number

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Protein Expressions of the Small GTPase Rho Proteins in Pterygial Tissue and Leukocytes of Patients with Pterygium
Saracaloglu A, et al.
Proceedings, 2(25), 1571-1571 (2018)
Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation
Dai J and Luftig MA
Journal of Immunology, ji1701473-ji1701473 (2018)
Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Lechuga A, et al.
Bio-protocol, 8(1), 1571-1571 (2018)
The blockade of lipophagy pathway is necessary for docosahexaenoic acid to regulate lipid droplet turnover in hepatic stellate cells
Qiu S, et al.
Biomedicine and Pharmacotherapy, 109, 1841-1850 (2019)
Does calcium disodium EDTA slow CKD progression?
Weaver VM, et al.
American Journal of Kidney Diseases, 60(4), 503-506 (2012)

Protocolli

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

Domande

1–2 di 2 domande  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Utile?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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