The molecular weight of this enzyme has not been analyzed. The enzymatic assay states a solubility of 1 mg/ ml in water.
Immediately prior to pipetting into cuvette, prepare a solution at 1 mg / mL in cold purified water. Place on ice.
This solution is stable for a maximum of thirty minutes.
F0628
Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach)
lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase
Sinonimo/i:
Ferridoxin-NADP+ oxidoreductase
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1 unit | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 141,00 € |
5 units | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 483,00 € |
10 units | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 777,00 € |
Informazioni su questo articolo
Numero CAS:
Numero CE:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
eCl@ss:
32160702
Specific activity:
≥15 units/mg solid
141,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Servizio Tecnico
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Permettici di aiutartiform
lyophilized powder
Quality Segment
specific activity
≥15 units/mg solid
foreign activity
NADH diaphorase activity ≤10 units/mg solid
storage temp.
−20°C
General description
Substrate Specificity
2 reduced [2Fe-2S]-[ferredoxin] + NADP+ + H+ = 2 oxidized [2Fe-2S]-[ferredoxin] + NADPH
2 reduced [2Fe-2S]-[ferredoxin] + NADP+ + H+ = 2 oxidized [2Fe-2S]-[ferredoxin] + NADPH
Ferredoxin-NADP+ Reductase is a ferredoxin-soluble partner with a molar ratio to PSI of 0.9 and 3.
Application
Ferredoxin-NADP+ Reductase was used in in vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes. It was also used to regulate glyceraldehyde-3-phosphate dehydrogenase.
Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach) has been used to test the effect of potential protein partners on the activity of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH)−CP12 preparation. It has also been used in standard assay conditions for phycocyanobilin:ferredoxin oxidoreductase (PcyA) enzyme assay.
Biochem/physiol Actions
Ferredoxin-NADP+ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.
Ferredoxin-NADP+ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.
Other Notes
One unit will reduce 1.0 millimole of cytochrome C per min at pH 7.5 at 25 °C in the presence of spinach ferredoxin and NADP.
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Questo articolo | ||
|---|---|---|
| description lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase | description lyophilized powder, Ferredoxin ≥15 wt. % | description buffered aqueous solution |
| specific activity ≥15 units/mg solid | specific activity - | specific activity - |
| form lyophilized powder | form lyophilized powder | form buffered aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| foreign activity NADH diaphorase activity ≤10 units/mg solid | foreign activity - | foreign activity - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 |
Classe di stoccaggio
11 - Combustible Solids
WGK
WGK 3
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
DPI (dispositivi di protezione individuale)
Eyeshields, Gloves, type N95 (US)
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Hi, how can I prepare a stock solution with the 1 U vial? It's not very clear for me in the specification sheet. I need a 1 uM final concentration of reductase for my assay, how do I prepare a stock solution of it?
1 risposta-
Utile?
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Hi there, could you please provide the specific methodology to extract this lyophilized powder from the vial? I didn't know how to extract this ferredoxin-NADP+ reductase from the vial
1 risposta-
The vials contain less than 1mg solid so reconstituting the whole vial is recommended.
Utile?
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