OGS1366
PSF-CMV-PURO-COOH-FRCFP - C-TERMINAL CFP TAG MAMMALIAN PLASMID
plasmid vector for molecular cloning
Sinonimo/i:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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Informazioni su questo articolo
NACRES:
NA.85
UNSPSC Code:
12352200
Promoter:
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
Promoter activity: constitutive
Promoter type: mammalian
Origin of replication:
pUC (500 copies)
Bacteria selection:
kanamycin
Reporter gene:
CFP
Peptide cleavage:
no cleavage
tag
cyan fluorescent protein (CFP) tagged
form
buffered aqueous solution
mol wt
size 6855 bp
bacteria selection
kanamycin
mammalian cells selection
puromycin
origin of replication
pUC (500 copies)
peptide cleavage
no cleavage
peptide tag location
C-terminal
promoter
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
reporter gene
CFP
shipped in
ambient
storage temp.
−20°C
General description
This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains a c-terminal non-aequorea based cyan/blue fluorescent protein (FrCFP) reporter tag that can be fused to a gene of interest to allow protein detection.
About the Cleavage Tag: This plasmid does not contain a protease cleavage site.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
About the Peptide Tag:This plasmid contains a c-terminal non-aequorea based cyan/blue fluorescent protein (FrCFP) reporter tag that can be fused to a gene of interest to allow protein detection.
About the Cleavage Tag: This plasmid does not contain a protease cleavage site.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.oxgene.com
Other Notes
OXGENE′s technology platforms and expert solutions accelerate the discovery, development and manufacture of cell and gene therapies. We work with you to express your gene of interest at scale, with high levels of purity. In addition, our integrated bioinformatics and high-throughput gene editing technology creates a streamlined workflow for automated cell line production. learn more at www.oxgene.com
Classe di stoccaggio
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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