As listed in the 'PROPERTIES' section, this kit is sufficient for approximately 200 tests. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/184/250/tp0300bul.pdf
TP0300
Total Protein Kit, Micro Lowry, Peterson′s Modification
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1 KIT
338,00 €
338,00 €
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Informazioni su questo articolo
Codice UNSPSC:
12352202
NACRES:
NA.32
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Permettici di aiutartiimpiego
sufficient for ~200 assays
1 of 4
Questo articolo | PE0230 | MOD50 | L3540 |
|---|---|---|---|
| usage sufficient for ~200 assays | usage - | usage sufficient for 50 reactions | usage - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
Descrizione generale
This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.
Applicazioni
Azioni biochim/fisiol
An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.
Caratteristiche e vantaggi
- Complete kit for protein assays
- Most widely cited protein assay
- Less protein to protein variation than other assays
- Contains reagents that can remove interfering substances
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
8A - Combustible corrosive hazardous materials
Classe di pericolosità dell'acqua (WGK)
WGK 3
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Protocolli
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
Contenuto correlato
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.
Metodi di quantificazione delle proteine, reagenti e saggi immunologici per misurare con precisione la concentrazione delle proteine in una varietà di campioni.
Instructions
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