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Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Organic & biomolecular chemistry (2015-01-03)

Sevnur Serim, Philipp Baer, Steven H L Verhelst

ABSTRACT

Activity-based probes (ABPs) are powerful tools for the analysis of active enzyme species in whole proteomes, cells or animals. Quenched fluorescent ABPs (qABPs) can be applied for real time imaging, allowing the visualization of dynamic enzyme activation by fluorescent microscopy. Unfortunately, qABPs are only available for a few enzymes. We here describe the design and synthesis of qABPs for serine proteases based on a phosphonate ester scaffold.

MATERIALI

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Descrizione del prodotto

Sigma-Aldrich

Acido acetico, glacial, ACS reagent, ≥99.7%

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Acido acetico, glacial, ReagentPlus^®, ≥99%

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Solfato di magnesio, anhydrous, ReagentPlus^®, ≥99.5%

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2-butanone, ACS reagent, ≥99.0%

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Acido acetico, glacial, ≥99.99% trace metals basis

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Acido acetico, suitable for HPLC

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Bromuro di litio, ReagentPlus^®, ≥99%

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(+)- L-ascorbato di sodio, crystalline, ≥98%

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Solfato di magnesio, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99.5%

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Bromuro di litio, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99%

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Di-tert-butyl dicarbonate, ReagentPlus^®, 99%

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(+)- L-ascorbato di sodio, BioXtra, ≥99.0% (NT)

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Copper(I) bromide, 98%

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Solfato di magnesio, puriss. p.a., drying agent, anhydrous, ≥98.0% (KT), powder (very fine)

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USP

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Supelco

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