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Quantitative determination of linoleic acid in infant formulas.

International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition (1991-01-01)
G Bellomonte, S Giammarioli, R Terilli
ABSTRACT

The aim of this paper is to develop a gas chromatographic method for the quantitative determination of linoleic acid in adapted, preterm, follow-up and special infant formulas. For sample preparation, lipids were extracted with a Soxhlet extractor after acid hydrolysis, and methyl esters of fatty acids were prepared in a sealed vial with hydrochloric acid in methyl alcohol and analysed using a packed polar column (SP 2330). Triheptadecanoin was chosen as internal standard. The method yielded reliable and repeatable results for all the examined formulas.

MATERIALI
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Supelco
SP®-2330 Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.20 μm
Supelco
SP®-2330 Capillary GC Column, L × I.D. 60 m × 0.25 mm, df 0.20 μm
Supelco
SP®-2330 Capillary GC Column, L × I.D. 30 m × 0.32 mm, df 0.20 μm
Supelco
SP®-2330 Capillary GC Column, L × I.D. 15 m × 0.25 mm, df 0.20 μm
Supelco
SP®-2330 Capillary GC Column, L × I.D. 60 m × 0.32 mm, df 0.20 μm