We offer over 200 modifications for both DNA and RNA oligonucleotides and probes. We can attach fluorescent dyes to oligos at the 5'-end, 3'-end and internal positions. Our product offering includes common dye modifiers such as 6-FAM™, HEX™, and TET™, as well as exotic modifications.
In addition to standard fluorescent modifications, we have the capabilities to provide Dual-Labeled Probes including Molecular Beacons, LightCycler® Probes, and Scorpions® Probes.
Modification Categories
| Fluorescent | Positions | Excitation Maximum (nm) | Emission Maximum (nm) | Fluorescent Color | |
|---|---|---|---|---|---|
| 6-FAM™ (fluorescein) | 5' End, 3' End | 495 | 520 | Green | |
| ROX™ | 5' End, 3' End | 586 | 610 | Orange | |
| Cyanine 3 | 5' End, 3' End | 549 | 566 | Green-Yellow | |
| Cyanine 5 | 5' End, 3' End | 646 | 669 | Red | |
| Cyanine 5.5 | 5' End | 675 | 694 | Red | |
| 6-FAM dT | 5' End, Internal | 495 | 520 | Green | |
| HEX™ | 5' End | 535 | 556 | Green | |
| JOE™ | 5' End, 3' End | 529 | 555 | Green | |
| 6-Carboxy-rhodamine 6G™ | 5' End, 3' End | 524 | 550 | Green | |
| TAMRA | 3' End | 557 | 583 | Green-Yellow | |
| TAMRA NHS Ester | 5' End | 557 | 583 | Yellow | |
| TET™ | 5' End | 521 | 536 | Green | |
| TxRd (Sulforhodamine 101-X) | 5' End, 3' End | 597 | 616 | Red-Orange | |
| A488 (Sulfonated Fluorescein 488) | 5' End, 3' End | 495 | 519 | Green | |
| WellRED D2-PA | 5' End | 750 | 770 | Red-Infrared | |
| WellRED D3-PA | 5' End | 685 | 706 | Red | |
| WellRED D4-PA | 5' End | 650 | 670 | Red | |
| Attachment | Positions | Purifications | |||
| Amine | |||||
| DMS(O)MT-Amino-Modifier-C6 | 5' End | Cartridge, HPLC | |||
| Amino-Modifier-C3-TFA | 5' End | Desalt, HPLC | |||
| Amino-Modifier-C12 | 5' End | Cartridge, HPLC | |||
| Amino-Modifier-C6-TFA | 5' End | Desalt, PAGE | |||
| Amino-dT | 5' End | Cartridge, HPLC | |||
| Amino-Modifier-5 | 5' End | Cartridge, HPLC | |||
| Amino-Modifier-C2-dT | Internal | Desalt, Cartridge, HPLC, PAGE | |||
| Amino-Modifier-C6-dT | Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 3'-Amino-Modifier-C7 | 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Glycerol | |||||
| Glyceryl | 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Phosphate | |||||
| Phosphate | 3', 5' End | Desalt, PAGE (5') Desalt, Cartridge, HPLC, PAGE (3') | |||
| Phosphate II | 5' End | Cartridge, HPLC | |||
| Thiol | |||||
| 5'-Thiol-Modifier C6 S-S | 5' End, Internal | Cartridge, HPLC | |||
| 3'-Thiol-Modifier-C3 S-S | 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Binding | Positions | Purifications | |||
| Biotin | |||||
| 5'-Biotin | 5' End | Desalt, Cartridge, HPLC, PAGE | |||
| Biotin | Internal | HPLC, PAGE | |||
| Biotin-dT | 5' End, Internal | HPLC, PAGE | |||
| Biotin-TEG | 5' End, Internal | Cartridge, HPLC | |||
| 3'-Biotin-TEG | 3' End | HPLC, PAGE | |||
| Digoxigenin | |||||
| Digoxigenin | 5' End, 3' End | HPLC | |||
| Dinitrophenol | |||||
| 2,4-Dinitrophenyl-TEG | 5' End, Internal | HPLC | |||
| Spacer | Positions | Purifications | |||
| Spacer | |||||
| Spacer 9 | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| Spacer 12 | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| Spacer 18 | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| Spacer C3 | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 3'-Spacer-C3 | 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Analog | Positions | Purifications | |||
| Chain Terminating | |||||
| 3'-dA | 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| 3'-dG | 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| 3'-dC | 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| 3'-dT | 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Chemotherapeutic | |||||
| Cytosine Arabinoside (araC) | Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 2',3'-dideoxycytidine (ddC) | 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Contamination Prevention | |||||
| Ribo U | 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Crosslinking | |||||
| 8-Br-dA | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 8-Br-dG | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 5-Br-dC | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 5-Br-dU | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 5-F-dU | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 5-I-dC | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 5-I-dU | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| Damage and Repair | |||||
| 2'-Deoxypseudouridine | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 5,6-Dihydro-dT | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 5,6-Dihydro-dU | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 5-OH-dC | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 5-OH-dU | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 8-Oxo-dA | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 8-Oxo-dG | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| Thymidine Glycol | 5' End, Internal | PAGE | |||
| TMP-F-dU, F-dC | Internal | Desalt, Cartridge, HPLC, PAGE | |||
| dUracil | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Degenerate | |||||
| 2'-Deoxynebularine | 5' End, Internal | Cartridge, HPLC, PAGE | |||
| Derivative K (dK) | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Derivative P (dP) | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Inosine | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| 5-Nitroindole | 5' End, Internal, 5' End | Desalt, Cartridge, HPLC, PAGE | |||
| 3-Nitropyrrole | 5' End, Internal, 3' End | Desalt (Internal only), Cartridge, HPLC, PAGE | |||
| Duplex Stabilizing | |||||
| 2,6-Diaminopurine | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 5-Me-dC | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| Fluorescent | |||||
| 2-Aminopurine | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| Etheno-dA | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| Hybridization Specificity | |||||
| Locked Nucleic Acid | 5' End, Internal, 3' End | Cartridge, HPLC, PAGE | |||
| Methylation Mutagenesis | |||||
| N6-Me-dA | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| O6-Me-dG | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| O4-Me-dT | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| No Base | |||||
| dSpacer | Internal | Desalt, Cartridge, HPLC, PAGE | |||
| siRNA Guide Strand Selection | |||||
| 5'-OMe-dT | 5' End | Desalt, HPLC, PAGE | |||
| Structure and Function | |||||
| 7-Deaza-dA | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 7-Deaza-dG | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 7-Deaza-dX | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| 7-Deaza-8-Aza-dA | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | |||
| dA-5' (Inverse Oligo) | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| dG-5' (Inverse Oligo) | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| dC-5' (Inverse Oligo) | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| dT-5' (Inverse Oligo) | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Translation Termination | |||||
| Puromycin | 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Intercalation | Positions | Purifications | |||
| Acridine | |||||
| Acridine | Internal | Desalt, Cartridge, HPLC, PAGE | |||
| Psoralen | |||||
| C2 | 5' End | Desalt, Cartridge, HPLC, PAGE | |||
| C6 | 5' End | Desalt, Cartridge, HPLC, PAGE | |||
| Antisense | Positions | Purifications | |||
| Cholesterol | |||||
| Cholesteryl-TEG | 5' End, Internal | Cartridge, HPLC | |||
| 3'-Cholesteryl-TEG | 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Methyl RNA | |||||
| 2'-OMe-RNA A | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| 2'-OMe-RNA G | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| 2'-OMe-RNA C | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| 2'-OMe-RNA U | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Methoxyethyl RNA | |||||
| 2'-MOE-RNA A | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| 2'-MOE-RNA G | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| 2'-MOE-RNA C | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| 2'-MOE-RNA U | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Fluoro RNA | |||||
| 2'-F-RNA A | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| 2'-F-RNA G | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| 2'-F-RNA C | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| 2'-F-RNA U | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| Thiophosphate | |||||
| Thiophosphate (S-Oligo) | 5' End, Internal, 3' End | Desalt, Cartridge (5' End, Internal) Desalt, Cartridge, HPLC, PAGE (3' End) | |||
| 2' → 5' Synthesis | |||||
| dA-5' | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| dG-5' | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| dC-5' | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
| dT-5' | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | |||
Phosphorothioates (or S-oligos)
Phosphorothioates (or S-oligos) are a variant of normal DNA in which one of the non-bridging oxygens is replaced by sulfur. The sulfurization of the internucleotide bond creates a molecule that is resistant to cellular nuclease degradation. Sulfur bonds can be introduced at the 5’, 3’, or internal positions.
Minimum Yield Guarantees | ||||
|---|---|---|---|---|
| Modified Oligos (O.D./µg)* | ||||
| Scale (µmole) | Desalt | Cartridge | HPLC | PAGE |
| 0.05 | 2/60 | 0.4/12 | 0.4/12 | 0.2/6 |
| 0.2 | 5/150 | 1/30 | 1/30 | 0.4/12 |
| 1.0 | 16/480 | 5/150 | 5/150 | 2/60 |
| 10 | 160/4,800 | NA | 52/1,560 | NA |
| 15 | 240/7,200 | NA | 76/2,280 | NA |
*Guarantees are for 20 mers or longer. Oligos shorter than 20 bases will have fewer ODs. Microgram amounts are calculated based on a 30 μg/OD value. If the μg/OD value is less than 30, the reported μg value will be proportionally less. Phosphorothioated yields are 70% of the above stated values. Post-synthesis modifications may yield 50% less than the above stated values.
| Non-Modified Phosphorothioates (S-Oligos) (O.D./µg )* | ||||
|---|---|---|---|---|
| Scale (µmole) | Desalt | Cartridge | HPLC | PAGE |
| 0.05 | 3.5/105 | 0.7/21 | 0.7/21 | 0.35/10.5 |
| 0.2 | 8.4/252 | 2.1/63 | 1.75/52.5 | 0.7/21 |
| 1.0 | 28/840 | 8.4/252 | 9.1/273 | 3.5/105 |
| 10 | 280/8,400 | NA | 91/2,730 | NA |
| 15 | 420/12,600 | NA | 133/3,990 | NA |
Shipping Schedule | ||||
|---|---|---|---|---|
| Purification | Desalt | Cartridge | HPLC | PAGE |
| Standard Modifications | 5-7 days or less | 5-7 days or less | 5-7 days or less | Inquire |
| Post-Synthesis Modifications | 7-10 days | 7-10 days | 7-10 days | Inquire |
*For complete label license information, please visit sigmaaldrich.com/oligolicenses.
Materials
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