IP50

Protein G Immunoprecipitation Kit

sufficient for 50 assays

• Synonym(s): Immunoprecipitation kit
• NACRES: NA.32
• UNSPSC Code: 12352203

Properties

• usage: sufficient for 50 assays
• Quality Segment: 200
• technique(s): antibody protein purification: suitable, immunoprecipitation (IP): suitable
• storage temp.: 2-8°C

Description

Application

The kit is designed to allow maximal recovery of immunoprecipitates. It provides all the necessary reagents to perform immunoprecipitation from cell extracts of any protein to which a suitable antibody is available. Based on protein G, the kit binds to most commonly used antibodies. In addition, spin columns are provided to enable quick washes without the loss of protein G resin and thus protein yield is maximized.

Features and Benefits

• Minimal loss of antigen-antibody bound beads during washing.
• Minimal or no non-specific signals by increasing the stringency of the washing step.

Preparation Note

When preparing reagents, use ultrapure water (17M -cm)

Kit Components Also Available Separately

• S6546

  5 M Sodium chloride solution 15 mL

  SDS
• P3296

  Protein G Agarose 2 mL

  SDS
• 71736

  10% Sodium dodecyl sulfate solution 1 mL

  SDS

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Warning
• Hazard Codes: H226,H315,H319
• Precautionary Statements: P210 - P233 - P240 - P241 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
• Storage Class: 3 - Flammable liquids
• WGK: WGK 3