This product is provided as a lyophilized powder. When stored appropriately at -20ºC, the material is quite stable and not assigned an expiration or retest date. A warranty period of one year from the date of shipment applies. Following reconstitution, stock solutions remain active for one week at 2 – 8ºC. Solutions are most stable at pH 6.5, as a pH >7 will greatly reduce thrombin activity. Since thrombin solutions adsorb to glass, it is recommended to aliquot solutions in plastic tubes and store at –20 ºC for long-term storage. Various literature sources indicate solutions may be most stable when stored at -70ºC. The long term stability of this product in solution has not been established.
T8885
Thrombin from human plasma
lyophilized powder, Suitable for routine use in the thrombin time test
Synonym(s):
Factor IIa
Sign In to View Organizational & Contract Pricing
Select a Size
10 VIALS
¥17,850
25 VIALS
¥52,290
¥17,850
List Price¥35,700Save 50%Check Cart for Availability
Diagnostics Manufacturers should use alternative grade product: SRE0003
Skip To
biological source
human plasma
form
lyophilized powder
packaging
vial of ≥10 NIH units
impurities
HIV, hepatitis B and hepatitis C, tested negative
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... F2(2147)
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | T7009 | 605190-M | 1.12374 |
|---|---|---|---|
| Gene Information human ... F2(2147) | Gene Information human ... F2(2147) | Gene Information - | Gene Information - |
| biological source human plasma | biological source - | biological source human plasma | biological source bovine plasma |
| form lyophilized powder | form lyophilized powder | form lyophilized | form solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. - | UniProt accession no. - |
| Quality Level 300 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
General description
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.
Application
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study that compared efficacy and safety of low-molecular-weight heparins (LMWHs) to other anticoagulants as venous thromboembolism prophylaxis in major orthopedic surgery. [1]
Biochem/physiol Actions
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Preparation Note
When reconstituted with 1 ml H2O, one vial will contain 10 mg bovine serum albumin, 0.15 M NaCl and 0.05 M sodium citrate, pH 6.5
Analysis Note
The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Other Notes
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
related product
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
T8885-1VL:
T8885-PH:
T8885-BULK:
T8885-VAR:
T8885-25VL:
T8885PROC:
T8885-10VL:
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Diana M Sobieraj et al.
Pharmacotherapy, 32(9), 799-808 (2012-06-30)
To evaluate the comparative efficacy and safety of low-molecular-weight heparins (LMWHs) versus other anticoagulants as venous thromboembolism prophylaxis in major orthopedic surgery. Systematic review with meta-analysis of 37 randomized controlled trials. Patients undergoing total hip replacement, total knee replacement, or
Meihua Hao et al.
Reproductive biology and endocrinology : RB&E, 20(1), 85-85 (2022-06-07)
Women with endometriosis have been shown to have a reduced vagal tone as compared with controls and vagotomy promoted while vagus nerve stimulation (VNS) decelerated the progression of endometriosis in mice. Extensive research also has shown that the activation of
Nathalie Busschaert et al.
Chembiochem : a European journal of chemical biology, 23(6), e202100670-e202100670 (2022-01-06)
The thrombin binding aptamer (TBA) is a 15-mer DNA oligonucleotide (5'-GGT TGG TGT GGT TGG-3'), that can form a stable intramolecular antiparallel chair-like G-quadruplex structure. This aptamer shows anticoagulant properties by interacting with one of the two anion binding sites
Michael Hultström et al.
Biomedicines, 10(6) (2022-06-25)
Hypercoagulation and endothelial dysfunction play central roles in severe forms of COVID-19 infections, but the molecular mechanisms involved are unclear. Increased plasma levels of the inflammatory cytokine and TIE2 receptor antagonist Angiopoietin-2 were reported in severely ill COVID-19 patients. In
Shenghui Huang et al.
Reproductive sciences (Thousand Oaks, Calif.), 29(4), 1170-1187 (2022-02-01)
The development of more efficacious, non-hormonal therapeutics for endometriosis is still an unmet medical need begging to be fulfilled. Growing evidence indicates that endometriotic lesions are wounds undergoing repeated tissue injury and repair, and, as such, platelets play an important
Related Content
-
What is the stability of this material after Lyophilization? Can I save it in the -20^0C?
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service